> Identification of other Streptococcus Species
Identification of other Streptococcus Species: Streptococcus General Methods
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The reference used for compiling the methods in Section I is:
Murray, P.R., Baron, E. J., Jorgensen, J.J., Pfaller, M.A., and Yolken, R.H. Manual of Clinical Microbiology, 8th ed. ASM Press: Washington, DC, 2003.
The Streptococcus species identification methods in Section II were compiled by several individuals, including Lynn Shewmaker, Richard Facklam, and Lucia Teixeira.
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Section I.
- Accuprobe-Enterococcus Test
- Accuprobe-Pneumococcus Test
- Acid formation in carbohydrate broth
- Arginine Hydrolysis
- Bacitracin Test
- Bile-esculin Test
- Bile solubility Test
- CAMP Test
- Catalase Test
- Clindamycin test
- Esculin hydrolysis
- Gas from MRS broth
- Gram Stain
- Growth at 10 & 45C
- Hemolysis
- Hippurate hydrolysis
- Lancefield Group Antigen
- Leucine amino peptidase (LAP)
- Litmus Milk Test
- Motility
- 6.5% NaCl Tolerance Test
- Optochin
- Pigmentation
- Pyridoxal Requirement Test (Vitamin B6)
- Pyrrolidonlarylamindase (PYR)
- Pyruvate Utilization Test
- Satellite Test
- Starch hydrolysis
- Sucrose agar (extracellular polysaccharides)
- Sucrose broth (5%)
- Tellurite tolerance
- Urea hydrolysis
- Vancomycin test
- Voges-Proskauer
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Section II.
Identification Procedures for Catalase-Negative Gram-positive Cocci
| A. | General Description of the general identified in this lab | |
| Table 1. | Identification of gram-positive general Beta-hemolytic streptococci | |
| Table 2. | Identification and reporting of β-hemolytic streptococci | |
| Table 3. | Identification of atypical group A strep | |
| Table 4. | Identification of the S. anginous group | |
| Non-beta-hemolytic streptococci | ||
| Streptococcus pneumoniae | ||
| Table 5. | Identification non-β-hemolytic strep | |
| Table 6. | Characteristics of viridans streptococci | |
| Table 7. | Identification of nutritionally variant strep |
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| B. | Identification of Enterococcus and Vagococcus | |
| Table 8. | Identification of Enterococcus and Vagococcus species |
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| C. | Identification of Lactococcus species | |
| Table 9. | Identification of Lactococcus species |
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| D. | Identification of Gemella species | |
| Table 10. | Identification of Gemella species |
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| E. | Identification of Pediococcus and Tetragenococcus species | |
| Table 11. | Identification of Pediococcus and Tetragenococcus |
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| F. | Identification of Leuconostoc species | |
| Table 12. | Identification of Leuconostoc species |
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| G. | Identification of Aerococcus and Helococcus | |
| Table 13. | Identification of Aerococcus and Helococcus |
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| H. | Identification of Globicatella | |
| Table 14. | Identification of Globicatella |
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| I. | Identification of Dolosigranulum, Ignavigranum, Facklamia, and Alloicococcus | |
| Table 15. | Identification of Dolosigranulum, Ignavigranum, Facklamia, and Alloicococcus | |
| Table 16. | Rapid ID-32 for unusual gram positive bacteria |
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