Department of Health and Human Services

Centers for Disease Control and Prevention

CDC en Español

Search:

Home: CDC Streptococcus Lab > Protocols > Alternative MLST Primers for S. pyogenes and S. pneumoniae

Alternative MLST Primers for S. pyogenes and S. pneumoniae

 

Alternative MLST primers. We have found that the primers listed at www.mlst.net (exit site) often lie to close for accurate sequencing results for the first 10-40 bases of the target using our capillary sequencers. We have found these alternative primers useful. These generally lie about 40 bases further upstream and downstream of the target sequences than the ones described at www.mlst.net (exit site).

S. pneumoniae alternative MLST primers.

aroE-fwd TCCTATTAAGCATTCTATTTCTCCCTTC
aroE-rev ACAGGAGAGGATTGGCCATCCATGCCCACACTG

recA-fwd GAATGTGTGATTCAATAATCACCTCAAATAGAAGG
recA-rev TGCTGTTTCGATAGCAGCATGGATGGCTTCC

spi-fwd CGCTTAGAAAGGTAAGTTATGAATTT
spi-rev GAAGAGGCTGAGATTGGTGATTCTCGGCC

xpt-fwd TTAACTTTTAGACTTTAGGAGGTCTTATG
xpt-rev CGGCTGCTTGCGAGTGTTTTTCTTGAG

ddl-fwd TAAAATCACGACTAAGCGTGTTCTGG
ddl-rev AAGTAGTGGGTACATAGACCACTGGG

S. pyogenes alternative MLST primers

gkigas-fwd Length: 24 1 gctgattttgtgggaattggtatg
gkigas-rev Length: 23 1 gtgagcgtagaaattctcctgct

gtr-fwd Length: 26 1 ggaattgatttagacattatgccagg
gtr-rev Length: 22 1 cacaataacgccgccatccata

recpgas-fwd Length: 22 1 tgtccgcaccctatcaatggat
recgas-rev Length: 24 1 catctttcacaaggatatgttgcc

xptgas-fwd Length: 29 1 atgcagttacttgaagaacgcatcttaac
xptgas-rev Length: 24 1 gcctccaagaagtttagattacca

yqi-fwd Length: 27 1 cttttgcaggttatcacatgggtataa
yqi-rev Length: 27 1 aagcaatagctcctccattg acattaa

muri-fwd Length: 28 1 ctatggtcctagacccaaaaaacaaatt
muri-rev Length: 25 1 ggatttgctgttgtaaaaaagcggt

muts-fwd Length: 22 1 aggtcagatgttagaggctagg
muts-rev Length: 27 1. cccagttcatcaaatagaataagagag

Primers for Streptococcus dysgalactiae subsp. equisimilis and Streptococcus canis MLST.

Note that all primers, with the exception of yqiZ, target genes corresponding to the S. pyogenes MLST scheme described at http://spyogenes.mlst.net/.

Primer name	sequence	amplicon length (bp)
gki-fwd gki-rev	GCAGATTTTATTGGTATCGGTATGG GTGAACGTAAGAATTCTCCTGCTG	498
gtr-fwd gtr-rev	GGAATTGATTTAAACATTATGTCAGGAG CACAATAACGCCGCCATCCATA	450
murI-fwd murI-rev	TTATGGTCCAAGACCTGCTGAGC TTTCAGGACTTGCCGTTGTGTAAAA	438
mutS-fwd mutS-rev	AGGTCAGATGTTAGAGGCTAGG CCTAGTTCATCAAATAGAATAAGGGAA	405
recP-fwd recP-rev	TGTCCGCACCCTATCAATGGAT CATCTTTCACAAGGATATGTTGCC	459
xpt-fwd xpt-rev	ATGCAGTTACTTGAAGAACGCATCTTAAC GCCTCCAAGAAGTTTAGATTACCA	450
yqiZ-fwd yqiZ-rev	CAGATGCTTTTAACAATTACCACATGG CCCATTCACATTCACGATTTCTGG	434

 

Back to Top

• Email this page

• Printer-friendly version

Safer Healthier People

Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA 30333, U.S.A
Public Inquiries: 1-800-CDC-INFO (232-4636) / 1-888-232-6348 (TTY)