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Figure 1. Neighbor-joining tree for 264 base pairs (bp) of nucleoprotein
gene sequence (bp 1157–1420) for 44 rabies virus samples from Sri Lanka
compared with samples from other areas of Asia and Africa with uncontrolled
dog rabies. Samples were aligned with Pasteur rabies virus (GenBank accession
no. M13215) by using the PileUp program of the Wisconsin Package version
10 (Genetic Computer Group, 1999, Madison, WI). The phylogenetic analyses
were perfomed by using the DNADIST (Kimura two-parameter method), Neighbor
(Neighbor-joining method), SEQBOOT, and CONSENSE programs of the PHYLIP
package, version 3.5 (available from: URL: http://evolution.genetics.washington.edu/phylip.html).
The program TREEVIEW was used to obtain the graphic output (available
from: URL: http://taxonomy.zoology.gla.ac.uk/rod/treeview.html).
Australian bat lyssavirus (GenBank AF081020) and Duvenhage virus (GenBank
U22848) were used as outgroups (not shown). Bootstrap values >50% in
1,000 resamplings of the data are shown at nodes corresponding to the
different lineages and sample clusters. The nodes signifying highest order
clustering of rabies virus lineages (supported by bootstrap values >70%)
are numbered 1–7. The two clusters of Sri Lankan samples, designated by
mutations at nucleotides 1231 and 1408, are indicated as A and B. Inset
shows a similar tree achieved by analysis of complete nucleoprotein gene
sequence (1414 bp). All nonidentical Sri Lankan samples were submitted
to GenBank (AY138549–AY138564). The Table
lists sample collection information.
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