 |
 |
 |
 |
|
|
|
|
 |
|
|
 |
|
|
|
|
| |
|||||||||||||||||
|
|
|
|
|
|
|
|||||||||||
|
|||||||||||||||||
|
EID
Home | Ahead of Print | Past
Issues | EID Search | Contact
Us | Announcements | Suggested
Citation | Submit Manuscript
Volume 8, Number 12, December 2002 Induction of Inflammation by West Nile virus Capsid through the Caspase-9 Apoptotic Pathway Joo-Sung Yang,* Mathura P. Ramanathan,*1 Karuppiah
Muthumani,* Andrew Y. Choo,* Sung-Ha Jin,* Qian-Chun Yu,* Daniel S. Hwang,*
Daniel K. Choo,* Mark D. Lee,* Kesen Dang,* WaixingTang,* J. Joseph Kim,†
and David B. Weiner* |
|
|
|
 |
|
| Back to article | |
|
Figure 1. Construction and subcellular expression of West Nile virus (WNV)–NY1999 capsid (Cp) gene–expressing plasmid, pcWNV-Cp-DJY: a, Genomic organization of WNV-NY1999 (10,945 bp) is outlined based on the published (GenBank accession no. AF202541). b, Cloning strategy for WNVCp gene–expressing plasmid, pcWNV-Cp-DJY. c, In vitro translated and immunoprecipitated 35S-labeled WNV-Cp visualized by SDS-PAGE. WNV-Cp–specific protein synthesis was compared to control generated by the vector backbone pcDNA3.1 (-). d, Protein expression by Western blot analysis, of WNV-Cp expression in HeLa cells. Subcellular location of WNV-Cp protein, in HeLa cells transfected with pcWNV-CpWT (e,f) or pcWNV-Cp-DJY (g,h) plasmids. 16 h posttransfection, the cells were visualized by indirect immunofluorescence. Typical nuclear staining was observed with the cells expressing WNV-CpWT (e) compared to the cells expressing WNV-Cp-DJY (g). TUNEL assay on the WNV-Cp–transfected cells, indicating nuclear condensation (i) due to expression of capsid (j). |
|
|
|
|
|
EID Home | Top of Page | Ahead-of-Print | Past Issues | Suggested Citation | EID Search | Contact Us | Accessibility | Privacy Policy Notice | CDC Home | CDC Search | Health Topics A-Z |
|
|
This page last reviewed March 21, 2003 |
|
|
Emerging
Infectious Diseases Journal |
|