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Appendix Figure 2. Amino acid sequences of the small
anellovirus (SAV) obtained in the present study (arrows show accession nos.
DQ409192 to DQ409199). GI, TE, and GA were from healthy donors; FI, RI, BE, DF,
and DT were from hepatitis C patients. Evolutionary relationships between and
within the 3 anelloviruses, torquetenovirus (TTV), torquetenominivirus (TTMV),
and small anellovirus (SAV) are shown. Analysis is based on the 18 amino acid (aa)
sequences of SAV above (75–104 aa), 46 open reading frame 2 (ORF2) amino acid
sequences representative of the 5 TTV genogroups (96–104 aa), and 10 ORF2 amino
acid sequences of TTMV (92–95 aa). Branching pattern was obtained by the FastME
algorithm included in DAMBE software package (version 4.2.13). Bootstrap
resampling was used to test the robustness of the tree, and bootstrap values >800
of 1,000 replicates are shown at the major branch points. The tree was drawn by
the Treeview software (version 1.6.6). The ORF2 (110 aa) of porcine circovirus
2 was used as outgroup. Mean and range percentage amino acid distances within
each of the 3 anelloviruses are shown in parentheses next to the virus names.
Scale bar indicates the estimated number of amino acid substitutions per site.
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