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Volume 12, Number 7, July 2006 Replicon Typing of Plasmids Encoding Resistance to Newer β-LactamsAlessandra
Carattoli,* Vivi Miriagou,† Alessia Bertini,* Alexandra Loli,† Celine Colinon,‡
Laura Villa,* Jean M. Whichard,§ and Gian Maria Rossolini‡ |
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Figure. Restriction enzyme analysis of plasmids analyzed in this study. Numbers and letters above each lane indicate strain reference names as defined in the Table. M is the1-kb Plus DNA ladder (Gibco-BRL, Gaithersburg, MD, USA). Plasmids were transferred by conjugation or transformation in Escherichia coli K12 strains and purified by Midi-prep purification kit. Plasmids were digested with restriction endonuclease PstI and separated by agarose (1%) gel electrophoresis in 1× TBE buffer. DNA was stained with ethidium bromide and visualized under UV light. The replicons and the β-lactamase genes carried by each group of plasmids are indicated below the figure. |
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This page last reviewed June 16, 2006 |
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Emerging
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