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Figure. Changes in norovirus (NoV) genotypes on the basis of phylogentic
trees of nucleotide sequences of 146/Kunming/04/China. Trees were constructed
from partial nucleotide sequences of capsid and polymerase regions of
146/Kunming/04/China. 146/Kunming/04/China is boldface. Dashed
boxes indicate the maintenance of genotypes of reference NoV strains,
and solid boxes indicate the involvement of NoV genotypes with recombinant
NoV 146/Kunming/04/China. A phylogenetic tree with 100 bootstrap resamples
of the nucleotide alignment datasets was generated by using the neighbor-joining
method with ClustalX. The genetic distance was calculated by using the
Kimura 2-parameter method (PHYLIP). The scale indicates nucleotide substitutions
per position. The numbers in the branches indicate the bootstrap values.
Manchester strain was used as an outgroup strain for phylogenetic analysis.
The nucleotide sequence of NoV strain 146/Kunming/04/China had been submitted
to GenBank and has been assigned accession no. DQ304651. Reference NoV
strains and accession nos. used in this study are as follows: Manchester
(X86560), Toronto (U02030), Melksham (X81879), Camberwell (AF145896),
Leeds/90/UK (AJ277608), Lordsdale (X86557), Hawaii (U07611), Saitama U3
(AB039776), Saitama U4 (AB039777), and Miami/94/US (AF414410).
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