Volume 12, Number 4, April 2006

Discrimination between Highly Pathogenic and Low Pathogenic H5 Avian Influenza A Viruses

Sunchai Payungporn,* Salin Chutinimitkul,* Arunee Chaisingh,† Sudarat Damrongwantanapokin,† Bandit Nuansrichay,† Wasana Pinyochon,† Alongkorn Amonsin,* Ruben O. Donis,‡ Apiradee Theamboonlers,* and Yong Poovorawan*
*Chulalongkorn University, Bangkok, Thailand; †National Institute of Animal Health, Bangkok, Thailand; and ‡Centers for Disease Control and Prevention, Atlanta, Georgia, USA

Figure. Discrimination between highly pathogenic avian influenza (HPAI) and low pathogenic avian influenza (LPAI) by melting curve analysis based on real-time reverse transcription–polymerase chain reaction of the H5 HA gene with SYBR Green I fluorescent dye. The melting peaks of HPAI and LPAI were clearly separated. The cutoff value was set at 78.50°C (midpoint between HPAI and LPAI) and used to interpret the pathogenicity of unknown samples.

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This page last reviewed February 27, 2006

Emerging Infectious Diseases Journal
National Center for Infectious Diseases
Centers for Disease Control and Prevention