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Past Issue

Vol. 12, No. 2
February 2006

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Synopsis

Bartonella quintana Characteristics and Clinical Management

Cédric Foucault,* Philippe Brouqui,* and Didier Raoult*Comments
*Université de la Méditerranée, Marseille, France


Appendix Table. Available primers for Bartonella spp. amplification

Microorganism

Gene

Direction*

Primer

Sequence

Bartonella clarridgeiae

ribC

F

PBC5

TACATAACGAGCCAATT

B. clarridgeiae

ribC

R

PBC15

TAGCTTTAGAACAATATGGT

B. henselae

ribC

F

PBH-L1

GATATCGGTTGTGTTGAAGA

B. henselae

ribC

R

PBH-R1

AATAAAAGGTATAAAACGCT

B. bacilliformis

ribC

F

PBH-L1

GATATCGGTTGTGTTGAAGA

B. bacilliformis

ribC

R

PBB-R1

AAAGGCGCTAACTGTTC

B. quintana

ribC

F

PBH-L1

GATATCGGTTGTGTTGAAGA

B. quintana

ribC

R

PBQ-R1

AAAGGGCGTGAATTTTG

All Bartonella

ribC

F

PBH3

CCAAGTGCTACATAACCATC

All Bartonella

ribC

R

PBH4

CGGGTTGTTATTGCTCTTAC

All (except B. bacilliformis)

16s-23s spacer

F

16SF

AGAGGCAGGCAACCACGGTA

All (except B. bacilliformis)

16s-23s spacer

R

23S1

GCCAAGGCATCCACC

All Bartonella

16s-23s spacer

F

QHVE1

TTCAGATGATGATCCCAA

All Bartonella

16s-23s spacer

R

QHVE2

TTGGGATCATCATCTGAA

All Bartonella

16s-23s spacer

F

QHVE3

GATATATTCAGACATGTT

All Bartonella

16s-23s spacer

R

QHVE4

AACATGTCTGAATATATC

B. bacilliformis

16s-23s spacer

F

BABF

CTGGATCACCTCCTTTCTAA

B. bacilliformis

16s-23s spacer

R

BABR

ATGCCCTTAAGACACTTGAT

B. quintana

16s-23s spacer

F

BQF

CTCCACCATTTTAGGTCATC

B. quintana

16s-23s spacer

R

BQR

GGTTTTGAGAATTCCCTTGC

All Bartonella

16s-23s spacer

F

16s1

(C/T)CTTCGTTTCTCTTTCTTCA

All Bartonella

16s-23s spacer

R

16s2

GGATAAACCGGAAAACCTTC

All Bartonella

16s-23s spacer

F

16s3

(C/T)CTTCGTTTCTCTTTCTTCA

All Bartonella

16s-23s spacer

R

16s4

AACCAACTGAGCTACAAGCC

All Bartonella

16s-23s spacer

F

16s5

CTCTTTCTTCAGATGATGATCC

All Bartonella

16s-23s spacer

R

16s6

AACCAACTGAGCTACAAGCCCT

All Bartonella

ribC

F

BARTON-1F

TAACCGATATTGGTTGTGTTGAAG

All Bartonella

ribC

R

BARTON-2R

TAAAGCTAGAAAGTCTGGCAACATAACG

All Bartonella

16s

S

probe

ATTTGGTTGGGCACTCTAGGGG

All Bartonella

16s

Rp2a

ACGGCTACCTTGTTAGGACTT

All Bartonella

16s

F

357f

TACGGGAGGCAGCAG

All Bartonella

16s

R

357ra

CTGCTGCCTCCCGTA

All Bartonella

16s

F

536F

CAGCAGCCGCGGTAATAC

All Bartonella

16s

R

536R

GTATTACCGCGGCTGCTG

All Bartonella

16s

F

800F

ATTAGATACCCTGGTAG

All Bartonella

16s

R

800R

CTACCAGGGTATCTAAT

All Bartonella

16s

F

1050F

TGTCGTCAGCTCGTG

All Bartonella

16s

R

1050R

CACGAGCTGACGACA

All Bartonella

35KD

F

35KD1fa

GTCGCTAAAGGCTGATGA

All Bartonella

35KD

R

35KD2ra

GACTGATATCGTGCGTGTG

All Bartonella

35KD

F

35KDs1f

GGTACGACGACAGTAATTGTT

All Bartonella

35KD

R

35KDs2r

GATTTAAGAGATACCAACCA

All Bartonella

PAP

F

PAP1fa

CTTTAATGACGACTTCTGTT

All Bartonella

PAP

R

PAP4ra

CCGAAATCTGAGTAACGGTA

All Bartonella

PAP

R

PAP2r

CCCTAAATGTTTCAAGTTCA

All Bartonella

PAP

F

PAP3f

GCTGACAGAGAAGACGCAA

All Bartonella

groEL

F

BbHs233.p

CGTGAAGTTGCCTCAAAAACC

All Bartonella

groEL

R

BbHs1630.n

AATCCATTCCGCCCATTC

All Bartonella

rpoB

F

QVE1

TTCAGATGATGATCCCAAGC

All Bartonella

rpoB

R

QVE3

AACATGTCTGAATATATCTTC

All Bartonella

Bfp1

ATTAATCTGCAYCGGCCAGA

All Bartonella

Bfp2

ACVGADACACGAATAACACC

All Bartonella

Bfs3

TTACAAAAATCYGTTGATAC

All Bartonella

Bfs4

GTATCAACRGATTTTTGTAA

All Bartonella

ftsZ

F

BaftsZF

GCTAATCGTATTCGCGAAGAA

All Bartonella

ftsZ

R

BaftsZR

GCTGGTATTTCCAAYTGATCT

B. henselae, B. clarridgeiae

ftsZ

BhftsZ 1393.n

GCGAACTACGGCTTACTTGC

B. henselae, B. clarridgeiae

ftsZ

Bh ftsZ 1247.p

CGGTTGGAGAGCAGTTTCGTC

B. quintana

ftsZ

F

Bq ftsZseqF

GCACATATTCTTGATGAGAT

B. quintana

ftsZ

R

Bq ftsZseqR

CCCCTATCATCTCATCAAG

B. bacilliformis

ftsZ

F

Bb ftsZseqF

GCGCATGTTCTTAGTGAAAT

B. bacilliformis

ftsZ

R

Bb ftsZseqR

CCTGTATACGTGATGCATTT

All Bartonella

ftsZ

FTS1p

GCCTTCTCATCCTCAACTT

All Bartonella

ftsZ

FTS2p

CAGCCTCTTCACGATGTG

All Bartonella

pap31

PAPn1

TTCTAGGAGTTGAAACCGAT

All Bartonella

pap31

PAPn2

GAAACACCACCAGCAACATA

All Bartonella

pap31

PAPns2

GCACCAGACCATTTTTCCTT

All Bartonella

pap31

PAPns1

CAGAGAAGACGCAAAAACCT

All Bartonella

groEL

HSPps1

CAGAAGTTGAAGTGAAAGAAAA

All Bartonella

groEL

HSPps2

GCNGCTTCTTCACCNGCATT

All Bartonella

groEL

HSPps4

GCTGGNGGTGTTGCNGTTA

All Bartonella

groEL

HSPps3

GCTGTNGAAGANGGNATTGT

*F, forward; R, reverse.
   
     
   
Comments to the Authors

Please use the form below to submit correspondence to the authors or contact them at the following address:

Didier Raoult, Unité des Rickettsies CNRS UMR 6020, Faculté de Médecine, Université de la Méditerranée, 27 Bd Jean Moulin, 13385 Marseille, CEDEX 05, France; fax: 33-4-91-83-03-90; email: didier.raoult@medecine.univ-mrs.fr

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This page posted January 24, 2006
This page last reviewed January 24, 2006

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