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Figure. Diagnostic polymerase chain reaction (PCR) restriction
fragment length polymorphism analysis for Echinococcus multilocularis
(lanes 1–8, 2 specimens in parallel) and E. granulosus (lanes 9–12,
1 specimen). Lane M: Gene Ruler 100-bp DNA ladder; lane C: negative control
without DNA; lanes 1 and 2: amplification of E. multilocularis
DNA with Eg9 PCR; lanes 3 and 4: amplification of E. multilocularis
DNA with Eg9 PCR, followed by cleavage with enzyme CfoI; lanes
5 and 6: amplification of E. multilocularis DNA with Eg9 PCR, followed
by cleavage with enzyme RsaI; lanes 7 and 8: amplification of E.
multilocularis DNA with Eg16 PCR; lane 9: amplification of E. granulosus
DNA with Eg9 PCR; lane 10: amplification of E. granulosus DNA with
Eg9 PCR, followed by cleavage with enzyme CfoI; lane 11: amplification
of E. granulosus DNA with Eg9 PCR, followed by cleavage with enzyme
RsaI; lane 12: amplification of E. granulosus DNA with Eg16
PCR.
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