Volume 11, Number 7, July 2005

Influenza A H5N1 Replication Sites in Humans

Mongkol Uiprasertkul,* Pilaipan Puthavathana,* Kantima Sangsiriwut,* Phisanu Pooruk,* Kanittar Srisook,* Malik Peiris,† John M. Nicholls,† Kulkanya Chokephaibulkit,* Nirun Vanprapar,* and Prasert Auewarakul*
*Mahidol University, Bangkok, Thailand; and †University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China

Figure 2. A) Detection of H5 influenza viral RNA in lungs, intestines, and spleen by reverse transcription–polymerase chain reaction (RT-PCR). B) Strand-specific RT-PCR detected positive-stranded viral RNA only in lungs and intestines but not in spleen. +/–, total RNA; –, negative-stranded RNA; +, positive-stranded RNA. RT-PCR products of an infected cell culture pellet and supernatant are shown as a control for proper amplification of the specific strands (lower panel). C) Tumor necrosis factor-α (TNF-α) mRNA was detected by RT-PCR only in lung tissue of the patient but not in lung tissue from a healthy control.

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This page last reviewed June 8, 2005

Emerging Infectious Diseases Journal
National Center for Infectious Diseases
Centers for Disease Control and Prevention