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Figure 1. Clustering of 175 sequence types representing 411 isolates
with eBURST (25). This algorithm identifies
the founder of a complex or genogroup of related sequence types (ST) and
subsequent patterns of evolutionary descent. The primary founder, indicated
in blue, of a complex is defined as the ST with the largest number of
single locus variants (SLVs). Larger complexes may contain secondary founders
of additional lineages that have a number of SLVs of their own. These
secondary founders are indicated in yellow. Numbers correspond to ST numbers.
The area of each circle corresponds to the number of isolates of the ST.
All complexes (major and minor) are shown. In addition, 57 STs did not
group into any of the complexes and were considered singletons (STs 13,
15, 30, 31, 36, 37, 38, 39, 42, 51, 52, 58, 59, 62, 65, 67, 68, 70, 72,
73, 74, 83, 84, 86, 89, 95, 96, 98, 99, 100, 102, 105, 107, 108, 110,
112, 114, 116, 118, 126, 129, 131, 142, 144, 145, 152, 153, 155, 162,
163, 164, 167, 168, 169, 170, 171, 172). The "epidemic" genetic
complex-17 derived from secondary founder ST-17 is indicated. A measurement
of statistical confidence in each of the assigned primary founders is
made by a bootstrap resampling procedure (25).The
predicted primary founders of the complexes 22, 94, 1, and 69 have a bootstrap
value of 73%, 84%, 85%, and 59%, respectively.
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