Volume 11, Number 3, March 2005

Rapid Identification of Emerging Pathogens: Coronavirus

Rangarajan Sampath,* Steven A. Hofstadler,* Lawrence B. Blyn,* Mark W. Eshoo,* Thomas A. Hall,* Christian Massire,* Harold M. Levene,* James C. Hannis,* Patina M. Harrell,* Benjamin Neuman,† Michael J. Buchmeier,† Yun Jiang,* Raymond Ranken,* Jared J. Drader,* Vivek Samant,* Richard H. Griffey,* John A. McNeil,* Stanley T. Crooke,* and David J. Ecker*
*Ibis Therapeutics, Carlsbad, California, USA; and †The Scripps Research Institute, La Jolla, California, USA

Figure 1. Electrospray ionization Fourier transfer ion cyclotron resonance (ESI-FTICR) mass spectrum from the polymerase chain reaction (PCR) amplicons from the severe acute respiratory syndrome (SARS)-associated coronavirus obtained with the propynylated RNA-dependent RNA polymerase primer pairs. The electrospray ionization conditions separate the sense and antisense strands of the PCR products. Multiple charge states are observed across the m/z range shown. The inset shows an expanded view of the isotope envelope of the (M-27H+)^27- species. As enumerated in Table 1, the derived molecular masses for the amplicon strands are 27298.518 (±0.03) Da and 27125.542 (±0.03) Da, corresponding to an unambiguous base composition of A27G19C14T28/ A28G14C19T27 for the double-stranded amplicon, the composition expected for the SARS isolate.

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This page last reviewed February 17, 2005

Emerging Infectious Diseases Journal
National Center for Infectious Diseases
Centers for Disease Control and Prevention