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Dispatch
Diagnostic System for Rapid
and Sensitive Differential Detection of Pathogens
Thomas Briese,* Gustavo Palacios,* Mark Kokoris,† Omar Jabado,* Zhiqiang
Liu,* Neil Renwick,* Vishal Kapoor,* Inmaculada Casas,‡ Francisco Pozo,‡
Ron Limberger,§ Pilar Perez-Brena,‡ Jingyue Ju,* and W. Ian Lipkin*
*Columbia University, New York, New York, USA; †Qiagen Inc., Valencia,
California, USA; ‡Instituto de Salud Carlos III, Majadahonda, Madrid,
Spain; and §New York State Department of Health, Albany, New York, USA
Appendix
| Appendix
Table. Respiratory panel Mass Tag primers |
|
|
Target – Masscode FWD/REV
|
Name FWD
|
Sequence
|
Name REV
|
Sequence
|
Ref.
|
|
|
FLUAV-M 618/690
|
AM-U151
|
CATGGAATGGCTAAAGACAAGACC
|
AM-L397
|
AAGTGCACCAGCAGAATAACTGAG
|
(8)
|
|
FLUAV-N1 499/439
|
NA1-U1078
|
ATGGTAATGGTGTTTGGATAGGAAG
|
NA1-L1352
|
AATGCTGCTCCCACTAGTCCAG
|
(8)
|
|
FLUAV-N2 658/730
|
NA2-U560
|
AAGCATGGCTGCATGTTTGTG
|
NA2-L858
|
ACCAGGATATCGAGGATAACAGGA
|
(8)
|
|
FLUAV-HA1 650/590
|
HA1-U583
|
GGTGTTCATCACCCGTCTAACAT
|
HA1-L895
|
GTGTTTGACACTTCGCGTCACAT
|
(8)
|
|
FLUAV-HA2 662/539
|
H2A208U27
|
GCTATGCAAACTAAACGGAATYCCTCC
|
H2A559L26
|
TATTGTTGTACGATCCTTTGGCAACC
|
|
|
FLUAV-HA3-1 586/475
|
HA3-U115
|
GCTACTGAGCTGGTTCAGAGTTC
|
HA3-L375
|
GAAGTCTTCATTGATAAACTCCAG
|
(8)
|
|
FLUAV-HA5 646/395
|
HA5hum-u71
|
TTACTGTTACACATGCCCAAGACA
|
HA5hum-L147
|
AGGYTTCACTCCATTTAGATCGCA
|
|
|
FLUBV 698/598
|
BHA-U188
|
AGACCAGAGGGAAACTATGCCC
|
BHA-L347
|
CTGTCGTGCATTATAGGAAAGCAC
|
(8)
|
|
|
HCoV-SARS 527/666
|
CIID-28891F
|
AAGCCTCGCCAAAAACGTAC
|
CIID-29100R
|
AAGTCAGCCATGTTCCCGAA
|
|
|
HCoV-229E 670/558
|
Taq-Co22-418F
|
GGCGCAAGAATTCAGAACCA
|
Taq-Co22-636R
|
TAAGAGCCGCAGCAACTGC
|
|
|
HCoV-OC43 686/548
|
Taq-Co43-270F
|
TGTGCCTATTGCACCAGGAGT
|
Taq-Co43-508R
|
CCCGATCGACAATGTCAGC
|
|
|
|
RSV A 467/455
|
RSA-U1137
|
AGATCAACTTCTGTCATCCAGCAA
|
RSV-L1192
|
GCACATCATAATTAGGAGTATCAAT
|
(9)
|
|
RSV B 483/479
|
RSB-U1248
|
AAGATGCAAATCATAAATTCACAGGA
|
RSV-1318
|
TGATATCCAGCATCTTTAAGTATCTTTATAGTG
|
(9)
|
|
|
HMPV-1 718/654
|
MPV01.2
|
AACCGTGTACTAAGTGATGCACTC
|
MPV02.2
|
CATTGTTTGACCGGCCCCATAA
|
(10)
|
|
HMPV-2 718/654
|
MV-Can-U918
|
AAGTCCAAAGGCAGGRCTGTTATC
|
MV-Can-L992
|
CCTGAAGCATTRCCAAGAACAACAC
|
|
|
|
HPIV-1 566/357
|
HPIV1-U82
|
TACTTTTGACACATTTAGTTCCAGGAG
|
HPIV1-L167
|
CGGTACTTCTTTGACCAGGTATAATTG
|
|
|
HPIV-2 463/590
|
HPIV2-U908
|
GGACTTGGAACAAGATGGCCT
|
HPIV2-L984
|
AGCATGAGAGCYTTTAATTTCTGGA
|
|
|
HPIV-3 423/539
|
HPIV3-U590
|
GCTTTCAGACAAGATGGAACAGTG
|
HPIV3-L668
|
GCATKATTGACCCAATCTGATCC
|
|
|
HPIV 4A 622/606
|
HPIV4A-U191
|
AACAGAAGGAAATGATGGTGGAAC
|
HPIV4A-L269
|
TGCTGTGGATGTATGGGCAG
|
|
|
HPIV 4B 622/606
|
HPIV4B-U194
|
AGAAGAAAACAACGATGAGACAAGG
|
HPIV4B-L306
|
GTTTCCCTGGTTCACTCTCTTCA
|
|
|
|
Measles 578/562
|
MEA-U1103
|
CAAGCATCATGATYGCCATTCCTGG
|
MEA-L1183
|
CCTGAATCYCTGCCTATGATGGGTTT
|
|
|
Adenovirus 503/630
|
ADV2HEX2F
|
CCCMTTYAACCACCACCG
|
ADVHEX1R
|
ACATCCTTBCKGAAGTTCCA
|
(11)
|
|
Enterovirus 702/495
|
5UTR-U447
|
TCCTCCGGCCCCTGAATGCGGCTAATCC
|
5UTR-L541
|
GAAACACGGWCACCCAAAGTASTCG
|
|
|
HHV-1 443/706
|
HSV-U27
|
CCCGGATGCGGTCCAGACGATTAT
|
HSV-L121
|
CCCGCGGAGGTTGTACAAAAAGCT
|
|
|
HHV-3 515/471
|
VZV-U138
|
ACGTGGATCGTCGGATCAGTTGT
|
VZV-L196
|
TCGCTATGTGCTAAAACACGCGG
|
|
|
Legionella pneumophila 678/582
|
LegPneu-U149
|
GCATWGATGTTARTCCGGAAGCA
|
LegPneu-L223
|
CGGTTAAAGCCAATTGAGCG
|
|
|
Chlamydia pneumoniae 519/383
|
CLPM1
|
CATGGTGTCATTCGCCAAGT
|
CLPM2
|
CGTGTCGTCCAGCCATTTTA
|
(12)
|
|
Streptococcus pneumonia 714/694
|
SPPLY-U532
|
AGCGATAGCTTTCTCCAAGTGG
|
SPPLY-L606
|
CTTAGCCAACAAATCGTTTACCG
|
|
|
Haemophilus influenzae 734/726
|
HINF-U82
|
AAGCTCCTTGMATTTTTTGTATTAGAA
|
Hinf-L158
|
GCTGAATTGGCTTRGATACCGAG
|
|
|
Mycoplasma pneumoniae 602/614
|
MTPM1
|
CCAACCAAACAACAACGTTCA
|
MTPM2
|
ACCTTGACTGGAGGCCGTTA
|
(12)
|
|
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