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Figure 1. Angrem52 and Angrem104 appear to be paramyxovirus genes.
A) Gene positions of a generic paramyxovirus and predicted genome position
of Angrem104 (top), the phosphoprotein (P) gene, Angrem52 (bottom), the
matrix protein (M) and fusion protein (F) genes. A potential editing site
(nucleotides 783–795), which might allow production from the OPmV P gene
of V and W/D proteins, is shown in genomic (negative) sense aligned with
the proposed editing sites of Nipah virus (NC_002728) (1)
and Hendra viruses (NC_001906). The full-length P open reading frame (ORF)
was obtained by inserting an additional nucleotide in the reported Angrem104
sequence (see text). Angrem52 is predicted to be a "read-through"
product of the M and F genes of a novel paramyxovirus. The full-length
F ORF was obtained by making 5 changes to the reported Angrem52 sequence
(see text). Putative gene-end, intergenic (IG), and gene-start transcription
regulatory signals lying between OPmV M and F genes are shown aligned
to the corresponding signals from Nipah and Hendra virus (shown in genomic
sense [12]). B) The putative OPmV F protein
contains a fusion peptide. The sequences surrounding the F protein cleavage
sites, including most fusion peptides, of several paramyxoviruses, including
putative OPmV, were aligned by using the AlignX program of the Vector
NTI6 software package. The arrow indicates the cleavage site. Residues
in red are absolutely conserved. Residues in blue are conserved in most
sequences. C) Organization of the putative OPmV P gene, allowing translation
of P, V, W, and C ORFs. D) Alignment of cysteine-rich carboxy-termini
of the putative OPmV and Nipah virus V proteins. The conserved carboxy-terminal
regions of the V proteins were aligned by using the AlignX program of
the Vector NTI6 software package. Conserved residues are indicated in
red, except for conserved cysteines, which are in blue. Underlined residues
are conserved among all paramyxovirus V proteins.
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