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Figure. Unrooted maximum-likelihood phylogram for 16S-23S ribosomal
RNA gene intergenic spacer sequences of Borrelia miyamotoi s.l.,
B. lonestari, B. hermsii, and B. turicatae. Maximum
likelihood settings for version 4.10b of PAUP* (http://paup.csit.fsu.edu)
for equally weighted characters corresponded to Hasegawa-Kishino-Yano
model with transition/transversion ratio, nucleotide frequencies, proportion
of invariable sites, and gamma distribution shape parameter estimated
by maximum likelihood. Support for clades was evaluated by 100 bootstrap
replications using full-heuristic search, and values >50% are indicated
along branches. Solid and dashed scale bars indicate the number of substitutions
per site for the corresponding branches. The geographic origins of the
isolates are indicated. The GenBank accession numbers for IGS 16-S rRNA
gene intergenic spacer genotype sequences are as follows: B. miyamotoi
s.l. type 1 (AY363703), type 2 (AY363704), type 3 (AY363705), and type
4 (AY363706); B. lonestari type 1 (AY363707), type 2 (AY363708),
and type 3 (AY363709); B. hermsii type 1 (AY515265), type 2 (AY515266),
type 3 (AY515267), and type 4 (AY515269); B. turicatae type 1 (AY526494)
and type 2 (AY526495). WA, Washington State; CA, California; NM, New Mexico;
CO, Colorado; KS, Kansas; TX, Texas; IL, Illinois; MO, Missouri; NJ, New
Jersey.
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