Volume 10, Number 2, February 2004

Ultrastructural Characterization of SARS Coronavirus

Cynthia S. Goldsmith,* Kathleen M. Tatti,* Thomas G. Ksiazek,* Pierre E. Rollin,* James A. Comer,* William W. Lee,* Paul A. Rota,* Bettina Bankamp,* William J. Bellini,* and Sherif R. Zaki*
*Centers for Disease Control and Prevention, Atlanta, Georgia, USA

Figure 4. Immunogold and in situ hybridization (ISH) labeling of severe acute respiratory syndrome–associated coronavirus–infected cells. A) Cytoplasmic area that is relatively free of organelles (arrow). B) At higher magnification, these regions are shown to consist of ribosomelike and filamentous structures. Within these regions, C) viral proteins are detected by immunolabeling, using hyperimmune mouse ascitic fluid (12 nm gold), and D) ultrastructural ISH detects viral mRNA, genRNA, or both, by using a pool of riboprobes (6 nm gold). Bars, A,1 mm; B–D, 100 nm. Note: For a printable reproduction of this image, please see the PDF version.

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This page last reviewed March 2, 2004

Emerging Infectious Diseases Journal
National Center for Infectious Diseases
Centers for Disease Control and Prevention