Volume 10, Number 10, October 2004

Escherichia coli and Community-acquired Gastroenteritis, Melbourne, Australia

Roy M. Robins-Browne,*† Anne-Marie Bordun,*† Marija Tauschek,* Vicki R. Bennett-Wood,*† Jacinta Russell,† Frances Oppedisano,† Nicole A. Lister,* Karl A. Bettelheim,* Christopher K. Fairley,* Martha I. Sinclair,‡ and Margaret E. Hellard‡
*University of Melbourne, Melbourne, Victoria, Australia; †Murdoch Children's Research Institute, Melbourne, Victoria, Australia; and ‡Monash University, Melbourne, Victoria, Australia

Figure 3. Fluorescent actin staining (FAS) assay for attaching-effacing capacity of atypical enteropathogenic Escherichia coli (EPEC) strains with different patterns of adherence to HEp-2 cells. Fluorescent micrographs of HEp-2 cells (A and B) incubated with strains of atypical EPEC showing localized-like and aggregative adherence, respectively, and then reacted with fluorescein-labelled phalloidin. Note the foci of intense fluorescence (arrows) associated with adherent bacteria, which were also visualized by phase contrast microscopy of the same microscope fields (C and D). Magnification x1,000.

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This page last reviewed September 13, 2004

Emerging Infectious Diseases Journal
National Center for Infectious Diseases
Centers for Disease Control and Prevention