Title: Transferrin Receptor
Contact Number: 1-866-441-NCHS
Years of Content: 2009 - 2010
First Published: February, 2012
Access Constraints: None
Use Constraints: None
Geographic Coverage: National
Subject:Transferrin Receptor, Nutrition, Biochemistries
Record Source: NHANES 2009 - 2010
Survey Methodology: NHANES 2009 - 2010 is a stratified multistage probability sample of the civilian non-institutionalized population of the U.S.
Medium: NHANES Web site; SAS transport files
The objectives of this component are: 1) to provide data for monitoring secular trends in measures of nutritional status in the U.S. population; 2) to evaluate the effect of people's habits and behaviors such as physical activity and the use of alcohol, tobacco, and dietary supplements on people's nutritional status; and 3) to evaluate the effect of changes in nutrition and public health policies including welfare reform legislation, food fortification policy, and child nutrition programs on the nutritional status of the U.S. population. These data will be used to estimate deficiencies and toxicities of specific nutrients in the population and subgroups, to provide population reference data, and to estimate the contribution of diet, supplements, and other factors to serum levels of nutrients. Data will be used for research to further define nutrient requirements as well as optimal levels for disease prevention and health promotion.
Participants aged 1-5 years, both genders, and females 12 to 49 years,who do not meet any of the exclusion criteria, are eligible.
Serum specimens were processed, stored, and shipped to the Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention for analysis.
Detailed specimen collection and processing instructions are discussed in the NHANES Laboratory/Medical Technologists Procedures Manual (LPM). Vials are stored under appropriate frozen (–30°C) conditions until they are shipped to National Center for Environmental Health for testing.
Hitachi Mod P:
The method principle for measurement of soluble transferrin receptor (sTfR) is immuno-turbidimetry using Roche kits on the Hitachi Mod P clinical analyzer. Latex bound anti-sTfR antibodies react with the antigen in the sample to form an antigen/antibody complex. Following agglutination, this is measured turbidimetrically.
There were no changes (from the previous 2 years of NHANES) to lab methods or lab site but there was a change to the equipment. In 2007-2008 the Hitachi 912 chemistry analyser was used but in 2009-2010 the Hitachi Mod P chemistry analyzer was used.
Read the General Documentation of Laboratory Data file for detailed data processing and editing protocols. The analytical methods are described in the Analytic Notes for Data Users section below.
The NHANES quality assurance and quality control (QA/QC) protocols meet the 1988 Clinical Laboratory Improvement Act mandates. Detailed QA/QC instructions are discussed in the NHANES Laboratory/Medical Technologists Procedures Manual (LPM). Read the General Documentation of Laboratory Data file for detailed QA/QC protocols.
For the transferrin receptor analysis, in 2007-2008 the Roche Hitachi 912 immunoturbidimetric assay was used and in 2009-2010 the Roche Hitachi Mod P immunoturbidimetric method was used. A crossover study was performed between the Hitachi 912 and Mod P sTfR methods, but no adjustment of the data was necessary.
The analysis of NHANES laboratory data must be conducted with the key survey design and basic demographic variables. The NHANES Household Questionnaire Data Files contain demographic data, health indicators, and other related information collected during household interviews. The Household Questionnaire Data Files also contain all survey design variables and sample weights required to analyze these data. The Phlebotomy Examination file includes auxiliary information on duration of fasting, the time of day of the venipuncture, and the conditions precluding venipuncture. The Household Questionnaire and Phlebotomy Exam files may be linked to the laboratory data file using the unique survey participant identifier SEQN.
In cases, where the result was below the limit of detection, the value for that variable is the detection limit divided by the square root of two.
Exam sample weights should be used for analyses. Please refer to the Analytic Guidelines for further details on the use of sample weights and other analytic issues. The Analytic Guidelines are available on the NHANES website.
|Code or Value||Value Description||Count||Cumulative||Skip to Item|
|0.9 to 54.5||Range of Values||2909||2909|