Table of Contents

Locator Record

Title: Apolipoprotein B (ApoB_F )
Contact Number: 1-866-441-NCHS
Years of Content: 2009 - 2010
First Published: June, 2012
Revised: NA
Access Constraints: None
Use Constraints: None
Geographic Coverage: National
Subject:Apolipoprotein B
Record Source: NHANES 2009 - 2010
Survey Methodology: NHANES 2009 - 2010 is a stratified multistage probability sample of the civilian non-institutionalized population of the U.S.
Medium: NHANES Web site; SAS transport files

Component Description

The goals of this component are: 1) to monitor the prevalence and trends in major cardiovascular conditions and risk factors in the U.S.; and 2) to evaluate prevention and treatment programs targeting cardiovascular disease in the U.S.

The main element of the cardiovascular disease laboratory component in NHANES is blood lipid levels. Cardiovascular disease is the leading cause of death in the United States. The data will be used to monitor the status of hyperlipidemia and the success of the National Cholesterol Education Program.

Eligible Sample

Participants aged 12 years and older who were examined in the morning (AM) session were tested.

Description of Laboratory Methodology

In an immunochemical reaction, Apolipoprotein B in the human serum sample form immune complexes with specific antibodies. These complexes scatter a beam of light passed through the sample. The intensity of the scattered light is proportional to the concentration of Apolipoprotein B in the sample. The result is evaluated by comparison with a standard of known concentration.

Apolipoprotein B is the main protein component of LDL and accounts for approximately 95% of the total protein content of LDL. Apo B is necessary for the reaction with LDL receptors in the liver and on cell walls, and is thus involved in transporting cholesterol from the liver to the vessel cell. Elevated levels of Apo B are frequently found in patients with atherosclerotic vascular changes and are a risk factor for atheroscelerosis.

There no changes to the lab site, lab instrument or methodology. A detailed description of the laboratory method used can be found on the NHANES Web site. 

Data Processing and Editing

Blood specimens were processed, stored and shipped to University of Minnesota for analysis. Detailed specimen collection and processing instructions are discussed in the NHANES LPM. Read the LABDOC file for detailed data processing and editing protocols. The analytical methods are described in the Description of the Laboratory Methodology section.

Detailed instructions on specimen collection and processing can be found on the NHANES website.

Laboratory Quality Assurance and Monitoring

The NHANES quality assurance and quality control (QA/QC) protocols meet the 1988 Clinical Laboratory Improvement Act mandates. Detailed QA/QC instructions are discussed in the NHANES Laboratory/Medical Technologists Procedures Manual (LPM). Read the LABDOC file for detailed QA/QC protocols.

A detailed description of the quality assurance and quality control procedures can be found on the NHANES Web site. 

Analytic Notes

General Analytic Notes:

The analysis of NHANES laboratory data must be conducted with the key survey design and basic demographic variables. The Demographic file contains: status variables providing core information on the survey participant including examination status, demographic variables including age, gender, race etc., and interview and examination sample weight variables and survey design variables. The Questionnaire data files contain socio-economic data, health indicators, and other related information collected during household interviews. Certain sensitive data on respondents under 18 years of age (e.g. HPV typing results, sexual behavior variables) are not included in the public use files. These data may be requested as described in the NHANES guidelines. The Phlebotomy file includes auxiliary information such as the conditions precluding venipuncture. These files may be linked to the Laboratory data file using the unique survey participant identifier SEQN.

Sampling Weights

The analyst should use the special sampling weights in this file to analyze Apolipoprotein B (ApoB). 

Please refer to the NHANES Analytic Guidelines  and the on-line NHANES Tutorial  for further details on the use of sample weights and other analytic issues.

References

Codebook and Frequencies

SEQN - Respondent sequence number

Variable Name:
SEQN
SAS Label:
Respondent sequence number
English Text:
Respondent sequence number.
Target:
Both males and females 12 YEARS - 150 YEARS

WTSAF2YR - Fasting Subsample 2 Year MEC Weight

Variable Name:
WTSAF2YR
SAS Label:
Fasting Subsample 2 Year MEC Weight
English Text:
Fasting Subsample 2 Year MEC Weight
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 337218.92 Range of Values 3581 3581
. Missing 0 3581

LBXAPB - Apolipoprotein (B) (mg/dL)

Variable Name:
LBXAPB
SAS Label:
Apolipoprotein (B) (mg/dL)
English Text:
Apolipoprotein (B) (mg/dL)
Target:
Both males and females 12 YEARS - 150 YEARS
Hard Edits:
to
Code or Value Value Description Count Cumulative Skip to Item
16 to 229 Range of Values 3357 3357
. Missing 224 3581

LBDAPBSI - Apolipoprotein (B) (g/L)

Variable Name:
LBDAPBSI
SAS Label:
Apolipoprotein (B) (g/L)
English Text:
Apolipoprotein (B) (g/L)
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.16 to 2.29 Range of Values 3357 3357
. Missing 224 3581