Table of Contents

Locator Record

Title: Antibody to Mumps Virus (Surplus Sera) (SSMUMP_A )
Contact Number: 1-866-441-NCHS
Years of Content: 1999 - 2000
First Published: April, 2008
Revised: June, 2009
Access Constraints: None
Use Constraints: None
Geographic Coverage: National
Subject:Antibody to Mumps Virus
Record Source: NHANES 1999 - 2000
Survey Methodology: NHANES 1999 - 2000 is a stratified multistage probability sample of the civilian non-institutionalized population of the U.S.
Medium: NHANES Web site; SAS transport files

Component Description

Mumps IgG antibody testing of stored serum specimens from NHANES (1999-2000) was conducted to estimate the seroprevalence of mumps antibody in the US population in 1999-2000.

Eligible Sample

Participants aged 6-49 years from of NHANES 1999-2000 with stored sera.

Description of Laboratory Methodology

Mumps antibody testing was performed using enzyme-linked immunosorbent assays (ELISAs) to detect mumps IgG (Wampole Laboratories, Princeton, NJ).

Specimens were tested according to manufacture directions.

Index values were calculated for each specimen by dividing the OD value by the cutoff OD.

Interpretation of Index Value of OD Ratio
Interpretation Index Value of OD Ratio 
Negative Specimens ≤0.900
Equivocal Specimens 0.901-1.099
Positive Specimens ≥1.100

Data Processing and Editing

Data Access: All data are publicly available.

Laboratory Quality Assurance and Monitoring

Serum samples were tested using Wampole Mumps IgG Test (Ref. # 425901CE). These were tested on two lots, the first lot used 19 kits and second lot used 57 kits.

Samples that initially tested Indeterminate or negative were repeated. If a discrepancy (difference in result and a change in ISR of > 0.5) was found the sample was repeated one more time. The previous result that matched the final test was the one accepted.

Assay reproducibility was assessed by random selection of 10% of the total number of serum specimens tested and repeating the ELISA assay.

Analytic Notes

The specimen index ratio (ISR) should be linked to the sequence number for all data analyses.

References

Codebook and Frequencies

SEQN - Respondent sequence number

Variable Name:
SEQN
SAS Label:
Respondent sequence number
English Text:
Respondent sequence number.
Target:
Both males and females 6 YEARS - 49 YEARS

SSISR - Specimen Index ratio

Variable Name:
SSISR
SAS Label:
Specimen Index ratio
English Text:
Specimen Index ratio
Target:
Both males and females 6 YEARS - 49 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.026 to 8.604 Range of Values 4699 4699
. Missing 8 4707

SSMUMPS - Antibody to mumps

Variable Name:
SSMUMPS
SAS Label:
Antibody to mumps
English Text:
Antibody to mumps
Target:
Both males and females 6 YEARS - 49 YEARS
Code or Value Value Description Count Cumulative Skip to Item
1 Positive 4306 4306
2 Negative 229 4535
3 Indeterminate 164 4699
9 Quantity not sufficient 0 4699
. Missing 8 4707