National Health and Nutrition Examination Survey

1999 - 2000 Data Documentation, Codebook, and Frequencies

Antibody to Cytomegalovirus IgG (Surplus Sera) (SSCMV_A)

Data File: SSCMV_A.xpt

First Published: March, 2008

Last Revised: NA

Locator Record

Title: Antibody to Cytomegalovirus IgG (Surplus Sera) (SSCMV_A )
Contact Number: 1-866-441-NCHS
Years of Content: 1999 - 2000
First Published: March, 2008
Revised: NA
Access Constraints: None
Use Constraints: None
Geographic Coverage: National
Subject:Antibody to Cytomegalovirus IgG
Record Source: NHANES 1999 - 2000
Survey Methodology: NHANES 1999 - 2000 is a stratified multistage probability sample of the civilian non-institutionalized population of the U.S.
Medium: NHANES Web site; SAS transport files

Component Description

Cytomegalovirus (CMV) antibody testing of stored sera specimens from NHANES 1999-2004 was conducted to estimate the seroprevalence of CMV infection (CMV IgG) in participants aged 6 to 49 years. An optical density measure for IgG is also included in this data release as an estimate of antibody titer.

Eligible Sample

Participants aged 6 to 49 years from NHANES 1999-2004 with stored sera (N = 15,336).

Description of Laboratory Methodology

CMV specific IgG was measured with an ELISA by Quest International, Inc., Miami FL. Sera with values near the ELISA cutoff (approx 5.2% of total) were confirmed with a second ELISA assay by bioMerieux, Inc., Durham, NC. When the results from these 2 tests disagreed (approx. 2.7% of total), an IFA from Bion International, Inc., was used and the result of the IFA was given as the final result.

Optical Density for the IgG test is reported. Optical density was measured with the ELISA assay by Quest International, Inc. and approximately corresponds to antibody titer; a low and positive optical density indicates low antibody titer and a high optical density indicates high antibody titer.

Data Processing and Editing

Data were received after all the antibody testing was complete. The data were not edited.

Data Access: All data are publicly available.

Laboratory Quality Assurance and Monitoring

Commercial reagents were used for all CMV testing. All QC procedures recommended by the manufacturers were followed.

Analytic Notes

There are two variables:

SSCMV: CMV specific IgG results (1=Positive, 2=Negative,
3= Equivocal)

SSCMVOD: CMV IgG Optical Density from the ELISA by Quest International, Inc., Miami FL. Measured in AU/ml which stands for Arbitrary units/ml. Continuous variable - Values range from 0.048 to 3.001. Values reported as “out of range” (i.e. over the detectable range, > 3.00) were assigned values of 3.001.

References

• Joassin, L., and Reginster, M. “Elimination of nonspecific cytomegalovirus immunoglobulin M activities in the enzyme-linked immunosorbent assay by using anti-human immunoglobulin G”. Journal of Clinical Microbiology. 23:576-581. 1986.
• Peckham, C.S. “Cytomegalovirus in the neonate”. Journal of Antimicrobial Chemotherapy. 23:17-21. 1989.
• Starr, S.E., and Friedman, H.M. “Human Cytomegalovirus”, Chapter 65 in Manual of Clinical Microbiology, fourth edition, edited by E.H. Lennette, A. Balows, W.J. Hausler, Jr., and H.J. Shadomy, American Society for Microbilolgy, pp 711-719. 1985.

Codebook and Frequencies