Table of Contents

Locator Record

Title: Immunofluorescence and immunoprecipitation analyses of autoantibodies (NHANES Surplus Sera) (SSANA_A )
Contact Number: 1-866-441-NCHS
Years of Content: 1999 - 2000
First Published: June, 2011
Revised: February, 2012
Access Constraints: None
Use Constraints: None
Geographic Coverage: National
Subject:Autoantibodies by Immunofluorescence
Record Source: NHANES 1999 - 2000
Survey Methodology: NHANES 1999 - 2000 is a stratified multistage probability sample of the civilian non-institutionalized population of the U.S.
Medium: NHANES Web site; SAS transport files

Component Description

IgG autoantibody testing of a subsample stored serum of specimens from NHANES (1999–2000) was conducted to estimate the prevalence and specificities of selected autoantibodies in the US population in 1999-2000.

Eligible Sample

One third subsample of participants aged 12 years and older from NHANES 1999-2000 in the Dioxin subsample who had stored sera (total of N = ~5,000 from NHANES 1999-2004).

Description of Laboratory Methodology

Immunofluorescence analysis
Testing of IgG autoantibodies to human cellular antigens was performed by the HEp-2 cell immunofluorescence assay using slides from INOVA Diagnostics, San Diego, CA (Cat # 508100) following the manufacturer’s instructions.

The cellular pattern of staining and its staining intensity from 0 to 4+ was described using a reference gallery per below. The determination of autoantibody titers from 1:80 to 1:1280 was performed by serial dilution on samples that showed a 3+ or greater nuclear and/or cytoplasmic immunofluorescence pattern.

Immunoprecipitation analysis
Testing of IgG autoantibodies to human cellular antigens was performed on samples that showed a 3+ or greater immunofluorescence staining using immunoprecipitation assays by a standard method (Reeves WH,et al., 2006)

Sera that showed known autoantibody specificities based on visible immunoprecipitated protein bands consistent with the combination of bands and their estimated size per the table below were recorded. Immunoprecipitated ribonucleic acid bands on stained gels were also recorded when they were needed to confirm the specificity. When necessary, the size and the pattern of migration were compared with reference sera (Reeves WH,et al., 2006).

Data Processing and Editing

Data Access: All data are publicly available.

Laboratory Quality Assurance and Monitoring

Immunofluorescence analysis
Serum samples were tested using established protocols outlined by manufacturer. A set of CDC autoimmune reference sera (12 different specificities) (Chan EKL et al., 2007) and 2 healthy control sera were coded and tested in a blinded fashion two times to verify consistent results throughout screening test: towards the beginning and when approximately half of the samples were tested.

Samples that showed discrepant results were repeated.

More than every 50th sample (2% of the total from NHANES 1999-2004) was automatically repeated to assess assay precision issues. Additional samples were repeated for the following reasons:
1) Titering of 3+/4+ positive samples (repeat, n=692)
2) Randomly selected samples for titering (and repeat, n=1035)
3) Other repeats are performed whenever there is any doubt for accuracy or technical issues. Samples repeated with 2 independent staining = 895; 3 independent staining = 194; 4 independent staining = 4.

Immunoprecipitation analysis
Serum samples were tested using the standard immunoprecipitation protocol (Reeves WH,et al., 2006). A set of CDC autoimmune reference sera (12 specificities) and 2 healthy controls were tested in a blinded fashion two times to verify consistent results throughout screening test: towards the beginning of the study and when approximately half of the samples were tested.

Samples that showed common bands were repeated in parallel side-by-side fashion for comparison with each other and with known controls as needed.

Assay reproducibility was initially assessed by random selection of the ANA 3+ or 4+ sera number of serum specimens tested and repeating the immunoprecipitation assay. The percentage of samples that were repeated was actually 15% (103/692) of the sera tested by IP.   

Analytic Notes

The NHANES Survey Design:

The analysis of NHANES laboratory data should be conducted using the key survey design variables. The NHANES Demographic data files for each 2-year survey cycle contain core information on the survey participant including the variables for: interview and examination status; demographic variables including age, gender, race/ethnicity; NHANES interview and examination sample weight variables and the survey design variables for strata and PSU (SDMVSTRA and SDMVPSU, respectively) which are recommended to be used in variance estimation.

The NHANES Questionnaire data files contain socio-economic data, health indicators, and other related information collected during household interviews. The Phlebotomy examination data file includes auxiliary information on duration of fasting, the time of day of the venipuncture, and the conditions precluding venipuncture. The demographic, questionnaire and phlebotomy examination files may be linked to the laboratory data file using the unique survey participant identifier SEQN. Autoantibody immunofluorescence and immunoprecipitation (ANA) data was obtained for a total of three NHANES cycles (1999-2000; 2001-2002; & 2003-2004). The datasets were released as SSANA_A, SSANA_B, and SSANA_C.

Weighting:

Special subsample sample weights are required to analyze the NHANES ANA data. This is because the ANA data was analyzed using stored serum samples obtained from survey participants who had been previously selected for the NHANES 1999-2004 Dioxin subsample. The Dioxin subsample is a randomly selected 1/3 subsample of each 2-year NHANES cycle, therefore the Dioxin subsample weights should be used for ANA data statistical analysis.

For the analysis of a single 2-year NHANES data release (for example the NHANES 1999-2000 ANA data) the 2-year Dioxin subsample weights WTSPO2YR should be used. If an analysis is performed combining the NHANES 1999-2000 and 2001–2002 ANA data into a single 4-year dataset, then the 4-year Dioxin subsample weights WTSPO4YR should be used for analysis. The NHANES 1999–2000 and 2001-2002 Dioxin data files (LAB28POC and LAB28POC_B, respectively) contain the WTSPO2YR WTSPO4YR subsample weight variables. To construct Dioxin subsample weights combining the three NHANES survey years 1999-2000, 2001-2001 and 2003-2004, please see the current NHANES Tutorial section “Constructing Weights for Combined NHANES Survey Cycles”.

The NHANES Environmental Chemical Data Tutorial has additional information on environmental subsamples in the section “Key Concepts about the Environmental Chemical Subsample Files”.   Please refer to the NHANES Analytic Guidelines  and the on-line NHANES Tutorial  for further details on the use of sample weights and other analytic issues.

 

Immunofluorescence analysis 

A standard gallery of images showing the intensity of staining and the cellular patterns of reactivity is shown below and the following patterns were reported:

Nuclear patterns
1) homogeneous 2) speckles 3) centromere
4) nuclear dots 5) nuclear envelope 6) nucleolar

Cytoplasmic patterns
1) homogeneous 2) speckles 3) mitochondria-like
4) Golgi complex 5) centriole  6) GW Body
7) cytofilament 8) rods/rings 9) lysosomes

Mitotic cell patterns
1) homogeneous 2) speckles 3) spindle apparatus
4) mid-body 5) chromosomes

ANA staining intensity reference gallery Staining Signal Guidelines for Multiple Staining Patterns

 

 

Nuclear Patterns
 Nuclear staining  Images  Description of staining patterns
 Homogeneous  Homogeneous Uniform diffuse staining of the nucleus in interphase cells  Uniform diffuse staining of the nucleus in interphase cells
  Speckles  Speckles in the nucleus of interphase cells  Speckles in the nucleus of interphase cells
 Centromere  Discrete speckles (40-80/cell) in interphase cells and on metaphase plates  Discrete speckles (40-80/cell) in interphase cells and on metaphase plates
 Nuclear dots  Countable discrete speckles PML bodies, Cajal bodies (coiled bodies)  Countable discrete speckles PML bodies, Cajal bodies (coiled bodies)
 Nuclear envelope   Homogeneous nuclear staining with greater intensity at its outer rim  Homogeneous nuclear staining with greater intensity at its outer rim
 Nucleolar  Homogeneous or speckled staining of the nucleolus  Homogeneous or speckled staining of the nucleolus

 

 

Cytoplasmic Patterns
 Cytoplasmic staining Images   Description of staining patterns
 Homogeneous Uniform diffuse staining of the cytoplasm in interphase  Uniform diffuse staining of the cytoplasm in interphase cells
 Speckles  Speckles Speckled staining of the cytoplasm  Speckled staining of the cytoplasm
 Mitochondria-like  Coarse granular filamentous staining  Coarse granular filamentous staining
 Golgi complex  Discontinuous speckled or granular perinuclear staining  Discontinuous speckled or granular perinuclear staining
 Centriole  Distinct dots (2-4/cell) in cytoplasm and at the poles of mitotic spindle  Distinct dots (2-4/cell) in cytoplasm and at the poles of mitotic spindle
 GW body  Discrete dots in cytoplasm of interphase cells  Discrete dots in cytoplasm of interphase cells
 Cytofilament  Cytoskeleton staining with variable intensity interphase cells  Cytoskeleton staining with variable intensity interphase cells
 Rods/Rings  Distinct rod and ring structures throughout the cytoplasm  Distinct rod and ring structures throughout the cytoplasm
 Lysosomes  Distinct and bright speckled staining irregularly distributed in the cytoplasm  Distinct and bright speckled staining irregularly distributed in the cytoplasm
 

 

Mitotic cell patterns
 Mitotic cell staining Images  Description of staining patterns 
 Homogeneous   Uniform diffuse staining of the mitotic cytoplasm  Uniform diffuse staining of the mitotic cytoplasm
 Speckles  Speckles Numerous speckles in cytoplasm of mitotic cells  Numerous speckles in cytoplasm of mitotic cells
 Spindle Apparatus  The spindle fibers between the poles are stained in mitotic cells, associated  The spindle fibers between the poles are stained in mitotic cells, associated with cone-shaped decoration of the mitotic poles
 Mid-body   Staining of the intercellular bridge that connects daughter cells by the end of cell division, but before cell separation  Staining of the intercellular bridge that connects daughter cells by the end of cell division, but before cell separation
 Chromosomes  Diffuse staining of condensed chromatin in mitotic cells  Diffuse staining of condensed chromatin in mitotic cells

Immunoprecipitation analysis
A standard set of images defining the immunoprecipitation patterns of reactivity is shown below and the specific reactivities that are reported are shown in the table below. Those samples that did not show any of these reactivities were reported as negative.

 Analysis of protein components of autoantigens (12.5% SDS-PAGE). 35S-methionine labeled K562 cell extract was immunoprecipitated by prototype human autoimmune sera or normal human serum (NHS). Components of each autoantigen are indicated by red arrowheads.

Analysis of protein components of autoantigens (12.5% SDS-PAGE). 35S-methionine labeled K562 cell extract was immunoprecipitated by prototype human autoimmune sera or normal human serum (NHS). Components of each autoantigen are indicated by red arrowheads.

Analysis of protein components of autoantigens (8% SDS-PAGE).  35S-methionine labeled K562 cell extract was immunoprecipitated by prototype human autoimmune sera or normal human serum (NHS). Components of each autoantigen are indicated by red arrowheads.

Analysis of protein components of autoantigens (8% SDS-PAGE). 35S-methionine labeled K562 cell extract was immunoprecipitated by prototype human autoimmune sera or normal human serum (NHS). Components of each autoantigen are indicated by red arrowheads.

Analysis of RNA components of autoantigens. K562 cell extract from 107 cells was immunoprecipitated by prototype sera for anti-U3RNP, Jo-1, and normal human serum (NHS). RNA component was extracted and analyzed by urea-PAGE and silver staining. RNA components of each autoantigen are shown with arrowheads. Total RNA and the pattern by anti-Sm serum are shown as references.

Analysis of RNA components of autoantigens. K562 cell extract from 107 cells was immunoprecipitated by prototype sera for anti-U3RNP, Jo-1, and normal human serum (NHS). RNA component was extracted and analyzed by urea-PAGE and silver staining. RNA components of each autoantigen are shown with arrowheads. Total RNA and the pattern by anti-Sm serum are shown as references.

Identification of autoantibodies by protein and RNA immunoprecipitation
Autoantibody Specificity 35S-methionine IP protein analysis IP-RNA analysis silver staining Definition
U1RNP Y ND A characteristic set of UsnRNPs proteins, U1-70k, A (33kD), B’/B (29/28kD), C (22kD), D1/D2/D3 (16kD). E,F, and G without U5-200kD doublet
Sm Y ND A characteristic set of UsnRNPs proteins as above with U5-200kD doublet
Ribosomal P Y ND A characteristic set of proteins P0 (38kD), P1 (19kD), and P2 (17kD)
Ro60 (SS-A) Y ND 60kD protein
La (SS-B) Y ND 48kD protein
Su (ago2) Y ND A set of 100/102kD doublet and 200kD proteins
Replication protein A Y ND A set of 70kD, 32kD, and 14kD proteins
Jo-1 (histidyl-tRNA synthetase) (Y) Y 50kD protein and tRNA by RNA analysis
PL-7 (threonyl-tRNA synthetase) Y ND 80kD protein
PL-12 (alanyl-tRNA synthetase) Y ND 110kD protein
EJ (glycyl-tRNA synthetase) Y ND 75kD protein
OJ (isoleucyl-tRNA synthetase) Y ND 130kD, 150kD, 170kD proteins
SRP (signal recognition particle) (Y) Y 72/68kD, 54kD, 19kD, 14kD, 9kD proteins and 7SL RNA by RNA analysis
Ku Y ND 70kD and 86kD proteins
PM-Scl Y ND 110kD, 75kD, and a set of other proteins
Mi-2 Y ND 240kD, 150kD proteins and several other proteins
Topoisomerase I (Scl-70) Y ND 110kD protein with a smear toward higher molecular weight due to phosphorylation
RNA polymerase I/III Y ND A characteristic set of proteins. Two largest subunits of each (RNAP I: 190, 120kD, RNAP III (155, 135kD)
U3RNP (Fibrillarin) (Y) Y 34kD protein, nucleolar staining, and U3RNA by RNA analysis
NOR-90 Y ND 93/95kD thick protein doublet

IP: immunoprecipitation; Y: Identity can be confirmed; (Y) identity suggested but requires RNA-IP to confirm; ND: not done

References

Codebook and Frequencies

SEQN - Respondent sequence number

Variable Name:
SEQN
SAS Label:
Respondent sequence number
English Text:
Respondent sequence number.
Target:
Both males and females 12 YEARS - 150 YEARS

SSCYT - total cytoplasm intensity

Variable Name:
SSCYT
SAS Label:
total cytoplasm intensity
English Text:
total cytoplasm intensity
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 4 Range of Values 1713 1713
. Missing 0 1713

SSNUC - total nucleus intensity

Variable Name:
SSNUC
SAS Label:
total nucleus intensity
English Text:
total nucleus intensity
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 4 Range of Values 1713 1713
. Missing 0 1713

SSTOT - total ANA intensity

Variable Name:
SSTOT
SAS Label:
total ANA intensity
English Text:
total ANA intensity
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 4 Range of Values 1713 1713
. Missing 0 1713

SS80 - 1:80 dilution

Variable Name:
SS80
SAS Label:
1:80 dilution
English Text:
1:80 dilution
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 0 201 201
. Missing 1512 1713

SS160 - 1:160 dilution

Variable Name:
SS160
SAS Label:
1:160 dilution
English Text:
1:160 dilution
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 1 Range of Values 201 201
. Missing 1512 1713

SS320 - 1:320 dilution

Variable Name:
SS320
SAS Label:
1:320 dilution
English Text:
1:320 dilution
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 1 Range of Values 201 201
. Missing 1512 1713

SS640 - 1:640 dilution

Variable Name:
SS640
SAS Label:
1:640 dilution
English Text:
1:640 dilution
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 1 Range of Values 201 201
. Missing 1512 1713

SS1280 - 1:1280 dilution or higher

Variable Name:
SS1280
SAS Label:
1:1280 dilution or higher
English Text:
1:1280 dilution or higher
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 1 Range of Values 201 201
. Missing 1512 1713

SSNUCPOS - nuclear nucleolar positive

Variable Name:
SSNUCPOS
SAS Label:
nuclear nucleolar positive
English Text:
nuclear nucleolar positive
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 1 Range of Values 1713 1713
. Missing 0 1713

SSNUCSIG - nuclear nucleolar signal

Variable Name:
SSNUCSIG
SAS Label:
nuclear nucleolar signal
English Text:
nuclear nucleolar signal
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 4 Range of Values 1713 1713
. Missing 0 1713

SSNUHOMP - nuclear homogenous positive

Variable Name:
SSNUHOMP
SAS Label:
nuclear homogenous positive
English Text:
nuclear homogenous positive
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 1 Range of Values 1713 1713
. Missing 0 1713

SSNUHOMS - nuclear homogenous signal

Variable Name:
SSNUHOMS
SAS Label:
nuclear homogenous signal
English Text:
nuclear homogenous signal
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 4 Range of Values 1713 1713
. Missing 0 1713

SSNUSPP - nuclear speckles positive

Variable Name:
SSNUSPP
SAS Label:
nuclear speckles positive
English Text:
nuclear speckles positive
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 1 Range of Values 1713 1713
. Missing 0 1713

SSNUSPS - nuclear speckles signal

Variable Name:
SSNUSPS
SAS Label:
nuclear speckles signal
English Text:
nuclear speckles signal
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 4 Range of Values 1713 1713
. Missing 0 1713

SSNUCENP - nuclear centromere positive

Variable Name:
SSNUCENP
SAS Label:
nuclear centromere positive
English Text:
nuclear centromere positive
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 1 Range of Values 1713 1713
. Missing 0 1713

SSNUCENS - nuclear centromere signal

Variable Name:
SSNUCENS
SAS Label:
nuclear centromere signal
English Text:
nuclear centromere signal
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 3 Range of Values 1713 1713
. Missing 0 1713

SSNUDOTP - nuclear dots positive

Variable Name:
SSNUDOTP
SAS Label:
nuclear dots positive
English Text:
nuclear dots positive
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 1 Range of Values 1713 1713
. Missing 0 1713

SSNUDOTS - nuclear dots signal

Variable Name:
SSNUDOTS
SAS Label:
nuclear dots signal
English Text:
nuclear dots signal
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 4 Range of Values 1713 1713
. Missing 0 1713

SSNUENVP - nuclear envelope positive

Variable Name:
SSNUENVP
SAS Label:
nuclear envelope positive
English Text:
nuclear envelope positive
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 1 Range of Values 1713 1713
. Missing 0 1713

SSNUENVS - nuclear envelope signal

Variable Name:
SSNUENVS
SAS Label:
nuclear envelope signal
English Text:
nuclear envelope signal
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 2 Range of Values 1713 1713
. Missing 0 1713

SSCYHOMP - cytoplasmic homogeneous positive

Variable Name:
SSCYHOMP
SAS Label:
cytoplasmic homogeneous positive
English Text:
cytoplasmic homogeneous positive
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 0 1713 1713
. Missing 0 1713

SSCYHOMS - cytoplasmic homogeneous signal

Variable Name:
SSCYHOMS
SAS Label:
cytoplasmic homogeneous signal
English Text:
cytoplasmic homogeneous signal
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 0 1713 1713
. Missing 0 1713

SSCYSPP - cytoplasmic speckles positive

Variable Name:
SSCYSPP
SAS Label:
cytoplasmic speckles positive
English Text:
cytoplasmic speckles positive
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 1 Range of Values 1713 1713
. Missing 0 1713

SSCYSPS - cytoplasmic speckles signal

Variable Name:
SSCYSPS
SAS Label:
cytoplasmic speckles signal
English Text:
cytoplasmic speckles signal
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 3 Range of Values 1713 1713
. Missing 0 1713

SSCYMIP - cytoplasmic mitochondria-like positive

Variable Name:
SSCYMIP
SAS Label:
cytoplasmic mitochondria-like positive
English Text:
cytoplasmic mitochondria-like positive
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 1 Range of Values 1713 1713
. Missing 0 1713

SSCYMIS - cytoplasmic mitochondria-like signal

Variable Name:
SSCYMIS
SAS Label:
cytoplasmic mitochondria-like signal
English Text:
cytoplasmic mitochondria-like signal
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 3 Range of Values 1713 1713
. Missing 0 1713

SSCYGOP - cytoplasmic golgi complex positive

Variable Name:
SSCYGOP
SAS Label:
cytoplasmic golgi complex positive
English Text:
cytoplasmic golgi complex positive
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 1 Range of Values 1713 1713
. Missing 0 1713

SSCYGOS - cytoplasmic golgi complex signal

Variable Name:
SSCYGOS
SAS Label:
cytoplasmic golgi complex signal
English Text:
cytoplasmic golgi complex signal
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 4 Range of Values 1713 1713
. Missing 0 1713

SSCYCEP - cytoplasmic centriole positive

Variable Name:
SSCYCEP
SAS Label:
cytoplasmic centriole positive
English Text:
cytoplasmic centriole positive
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 1 Range of Values 1713 1713
. Missing 0 1713

SSCYCES - cytoplasmic centriole signal

Variable Name:
SSCYCES
SAS Label:
cytoplasmic centriole signal
English Text:
cytoplasmic centriole signal
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 4 Range of Values 1713 1713
. Missing 0 1713

SSCYGWP - cytoplasmic GW body positive

Variable Name:
SSCYGWP
SAS Label:
cytoplasmic GW body positive
English Text:
cytoplasmic GW body positive
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 1 Range of Values 1713 1713
. Missing 0 1713

SSCYGWS - cytoplasmic GW body signal

Variable Name:
SSCYGWS
SAS Label:
cytoplasmic GW body signal
English Text:
cytoplasmic GW body signal
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 3 Range of Values 1713 1713
. Missing 0 1713

SSCYCFP - cytoplasmic cytofilament positive

Variable Name:
SSCYCFP
SAS Label:
cytoplasmic cytofilament positive
English Text:
cytoplasmic cytofilament positive
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 1 Range of Values 1713 1713
. Missing 0 1713

SSCYCFS - cytoplasmic cytofilament signal

Variable Name:
SSCYCFS
SAS Label:
cytoplasmic cytofilament signal
English Text:
cytoplasmic cytofilament signal
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 4 Range of Values 1713 1713
. Missing 0 1713

SSCYRRP - cytoplasmic rods/rings positive

Variable Name:
SSCYRRP
SAS Label:
cytoplasmic rods/rings positive
English Text:
cytoplasmic rods/rings positive
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 1 Range of Values 1713 1713
. Missing 0 1713

SSCYRRS - cytoplasmic rods/rings signal

Variable Name:
SSCYRRS
SAS Label:
cytoplasmic rods/rings signal
English Text:
cytoplasmic rods/rings signal
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 4 Range of Values 1713 1713
. Missing 0 1713

SSCYLYP - cytoplasmic lysosomes positive

Variable Name:
SSCYLYP
SAS Label:
cytoplasmic lysosomes positive
English Text:
cytoplasmic lysosomes positive
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 1 Range of Values 1713 1713
. Missing 0 1713

SSCYLYS - cytoplasmic lysosomes signal

Variable Name:
SSCYLYS
SAS Label:
cytoplasmic lysosomes signal
English Text:
cytoplasmic lysosomes signal
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 3 Range of Values 1713 1713
. Missing 0 1713

SSMIHOMP - mitotic homogeneous positive

Variable Name:
SSMIHOMP
SAS Label:
mitotic homogeneous positive
English Text:
mitotic homogeneous positive
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 0 1713 1713
. Missing 0 1713

SSMIHOMS - mitotic homogeneous signal

Variable Name:
SSMIHOMS
SAS Label:
mitotic homogeneous signal
English Text:
mitotic homogeneous signal
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 0 1713 1713
. Missing 0 1713

SSMISPP - mitotic speckles positive

Variable Name:
SSMISPP
SAS Label:
mitotic speckles positive
English Text:
mitotic speckles positive
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 1 Range of Values 1713 1713
. Missing 0 1713

SSMISPS - mitotic speckles signal

Variable Name:
SSMISPS
SAS Label:
mitotic speckles signal
English Text:
mitotic speckles signal
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 4 Range of Values 1713 1713
. Missing 0 1713

SSMISAP - mitotic spindle apparatus positive

Variable Name:
SSMISAP
SAS Label:
mitotic spindle apparatus positive
English Text:
mitotic spindle apparatus positive
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 1 Range of Values 1713 1713
. Missing 0 1713

SSMISAS - mitotic spindle apparatus signal

Variable Name:
SSMISAS
SAS Label:
mitotic spindle apparatus signal
English Text:
mitotic spindle apparatus signal
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 4 Range of Values 1713 1713
. Missing 0 1713

SSMIMBP - mitotic mid-body positive

Variable Name:
SSMIMBP
SAS Label:
mitotic mid-body positive
English Text:
mitotic mid-body positive
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 1 Range of Values 1713 1713
. Missing 0 1713

SSMIMBS - mitotic mid-body signal

Variable Name:
SSMIMBS
SAS Label:
mitotic mid-body signal
English Text:
mitotic mid-body signal
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 4 Range of Values 1713 1713
. Missing 0 1713

SSMICHP - mitotic chromosomes positive

Variable Name:
SSMICHP
SAS Label:
mitotic chromosomes positive
English Text:
mitotic chromosomes positive
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 1 Range of Values 1713 1713
. Missing 0 1713

SSMICHS - mitotic chromosomes signal

Variable Name:
SSMICHS
SAS Label:
mitotic chromosomes signal
English Text:
mitotic chromosomes signal
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 4 Range of Values 1713 1713
. Missing 0 1713

SSU1RNP - U1 small nuclear ribonucleoprotein

Variable Name:
SSU1RNP
SAS Label:
U1 small nuclear ribonucleoprotein
English Text:
U1 small nuclear ribonucleoprotein
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 1 Range of Values 201 201
. Missing 1512 1713

SSSM - Sm antigen

Variable Name:
SSSM
SAS Label:
Sm antigen
English Text:
Sm antigen
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 0 201 201
. Missing 1512 1713

SSRIBOP - ribosomal P protein

Variable Name:
SSRIBOP
SAS Label:
ribosomal P protein
English Text:
ribosomal P protein
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 0 201 201
. Missing 1512 1713

SSROSSA - Ro/SS-A 60kDa antigen

Variable Name:
SSROSSA
SAS Label:
Ro/SS-A 60kDa antigen
English Text:
Ro/SS-A 60kDa antigen
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 1 Range of Values 201 201
. Missing 1512 1713

SSLASSB - La/SS-B antigen

Variable Name:
SSLASSB
SAS Label:
La/SS-B antigen
English Text:
La/SS-B antigen
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 1 Range of Values 201 201
. Missing 1512 1713

SSSUAR2 - Su antigen/Argonaute2 (Ago2) protein

Variable Name:
SSSUAR2
SAS Label:
Su antigen/Argonaute2 (Ago2) protein
English Text:
Su antigen/Argonaute2 (Ago2) protein
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 1 Range of Values 201 201
. Missing 1512 1713

SSRPA - replication protein A

Variable Name:
SSRPA
SAS Label:
replication protein A
English Text:
replication protein A
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 1 Range of Values 201 201
. Missing 1512 1713

SSJO_1 - Jo-1 antigen

Variable Name:
SSJO_1
SAS Label:
Jo-1 antigen
English Text:
Jo-1 antigen
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 0 201 201
. Missing 1512 1713

SSPL_7 - PL-7 antigen

Variable Name:
SSPL_7
SAS Label:
PL-7 antigen
English Text:
PL-7 antigen
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 0 201 201
. Missing 1512 1713

SSPL_12 - PL-12 antigen

Variable Name:
SSPL_12
SAS Label:
PL-12 antigen
English Text:
PL-12 antigen
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 0 201 201
. Missing 1512 1713

SSEJ - EJ antigen

Variable Name:
SSEJ
SAS Label:
EJ antigen
English Text:
EJ antigen
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 0 201 201
. Missing 1512 1713

SSOJ - OJ antigen

Variable Name:
SSOJ
SAS Label:
OJ antigen
English Text:
OJ antigen
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 0 201 201
. Missing 1512 1713

SSSRP - signal recognition particle

Variable Name:
SSSRP
SAS Label:
signal recognition particle
English Text:
signal recognition particle
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 0 201 201
. Missing 1512 1713

SSKU - Ku antigen

Variable Name:
SSKU
SAS Label:
Ku antigen
English Text:
Ku antigen
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 0 201 201
. Missing 1512 1713

SSPM_SCL - PM-Scl antigen

Variable Name:
SSPM_SCL
SAS Label:
PM-Scl antigen
English Text:
PM-Scl antigen
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 0 201 201
. Missing 1512 1713

SSMI_2 - Mi-2 antigen

Variable Name:
SSMI_2
SAS Label:
Mi-2 antigen
English Text:
Mi-2 antigen
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 0 201 201
. Missing 1512 1713

SSTOPOI - Topoisomerase I

Variable Name:
SSTOPOI
SAS Label:
Topoisomerase I
English Text:
Topoisomerase I
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 1 Range of Values 201 201
. Missing 1512 1713

SSRNAPOL - RNA polymerase I and III

Variable Name:
SSRNAPOL
SAS Label:
RNA polymerase I and III
English Text:
RNA polymerase I and III
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 0 201 201
. Missing 1512 1713

SSU3RNP - U3 small nuclear ribonucleoprotein

Variable Name:
SSU3RNP
SAS Label:
U3 small nuclear ribonucleoprotein
English Text:
U3 small nuclear ribonucleoprotein
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 0 201 201
. Missing 1512 1713

SSNOR_90 - nucleolar organizer region antigen 90kDa

Variable Name:
SSNOR_90
SAS Label:
nucleolar organizer region antigen 90kDa
English Text:
nucleolar organizer region antigen 90kDa
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 0 201 201
. Missing 1512 1713