Table of Contents

Locator Record

Title: PHPYPA Urinary Phthalates (PHPYPA )
Contact Number: 1-866-441-NCHS
Years of Content: 1999 - 2000
First Published: June, 2002
Revised: January, 2012
Access Constraints: None
Use Constraints: None
Geographic Coverage: National
Subject:Urinary Phthalates, Urinary Polycyclic Aromatic Hydrocarbons (PAHs), and Urinary Phytoestrogens
Record Source: NHANES 1999 - 2000
Survey Methodology: NHANES 1999 - 2000 is a stratified multistage probability sample of the civilian non-institutionalized population of the U.S.
Medium: NHANES Web site; SAS transport files

Component Description

Urinary Phthalates

Phthalate acid esters (phthalates) are used extensively as plasticizers in a wide range of applications such as children’s toys, food packaging, and medical supplies. Because some of these compounds are known to be estrogenic and have been associated with a host of health problems in rats, such as cancers and teratogenicity, governments in Europe and Japan have become increasingly concerned about levels in food packaging materials and children’s toys. Biomeasures of phthalates in humans is necessary to evaluate potential human health threats from exposure to these chemicals.

Urinary Polycyclic Aromatic Hydrocarbons (PAHs)

PAHs constitute a group of chemicals, which are formed during the incomplete combustion of coal, oil and gas, garbage, and other organic substances. These compounds require metabolic activation prior to their interactions with cellular macromolecules. PAHs are ubiquitous, thus exposure to them is widespread. In general, people are exposed to mixtures of PAHs, the sources of which include vehicle exhausts, asphalt roads, coal, coal tar, wild fires, agricultural burning, charbroiled foods, and hazardous waste sites. Although most of the data regarding the carcinogenicity of these compounds comes from rats and mice, epidemiologic studies have shown increased mortality due to lung and bladder cancer in humans exposed to coke-oven emissions, roofing-tar emissions, and cigarette smoke. PAHs enter the body quickly and easily by all routes of exposure and are readily and predominantly metabolized to hydroxylated metabolites as well as glucuronide metabolites. These metabolites are excellent indicators of exposure to the parent PAHs. While background level ranges of PAHs in air and water are known, the equivalent metabolite background levels in humans are not known. Because of increased epidemiologic data relating PAH exposure to cancer incidence, biomonitoring PAH metabolites in humans is very important.

Urinary Phytoestrogens

Many different plants produce compounds, called phytoestrogens that mimic or interact with estrogen. The major classes of phytoestrogens are lignans (present in flaxseed, carrots, berries, and grapes) and isoflavones (present in soybeans and other legumes). Biomeasures of phytoestrogens are necessary to establish reference ranges for these compounds and to evaluate their potential effects on human health.

Eligible Sample

Participants aged 6 years and older.

Description of Laboratory Methodology

Data Collection Methods

Urine specimens are processed, stored, and shipped to the Division of Environmental Health Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention for analysis.

Examination Protocol

Detailed specimen collection and processing instructions are discussed in the NHANES Laboratory/Medical Technologists Procedures Manual (LPM). Vials are stored under appropriate frozen (–20°C) conditions until they are shipped to National Center for Environmental Health for testing.

Analytic Methodology

Urinary Phthalates: Mono-Ethyl Phthalate, Mono-n-Butyl Phthalate, Mono-Cyclohexyl Phthalate, Mono- (2-Ethyl) Hexyl Phthalate, Mono-n-Octyl Phthalate, Mono-Benzyl Phthalate, and Mono-Isononyl Phthalate

Human urine samples were processed using enzymatic deconjugation of the glucuronides followed by solid-phase extraction. The eluate was concentrated, and the phthalate metabolites chromatographically resolved by reversed-phase HPLC, detected by APCI- tandem mass spectrometry (APCI-MS/MS), and quantified by isotope dilution. Assay precision was improved by incorporating 13C4- labeled internal standards for each of the seven analytes, as well as a conjugated internal standard to monitor deconjugation efficiency. This selective method allows for rapid detection of seven metabolites of commonly used dialkyl phthalates in human urine with limits of detection in the low parts per billion (ppb) range.

Urinary Polycyclic Aromatic Hydrocarbons (PAHs)

This method is used to assess human exposure to selected PAHs, a class of potential human carcinogens, by determining the concentrations of their mono-hydroxy metabolites in urine. Common routes of occupational exposure to PAHs may include work involving diesel fuels and coal tars through paving and roofing. Possible environmental exposures may include smoking, dietary, smog and forest fires. Threshold levels for carcinogenicity have not been determined for most PAHs. Application of this method to the National Health and Nutrition Examination Survey (NHANES) project will help determine the concentration range of these chemicals in residents in the United States.

The specific analytes measured in this method are monohydroxy-polycyclic aromatic hydrocarbons (OH-PAH). The procedure involves enzymatic hydrolysis of urine (to hydrolyze PAH conjugates), solid-phase extraction, derivatization, and analysis using capillary gas chromatography combined with high-resolution mass spectrometry (GC/HRMS). This method uses isotope dilution with 13C-labeled internal standards. Ions from each analyte and each 13C-labeled internal standard are monitored, and the abundances of each ion are measured. The ratios of these ions are used as criteria for evaluating the data. By evaluating the concentrations of these analytes in urine, an assessment of human exposure to the respective PAH can be obtained.

Urinary Phytoestrogens: Daidzein, Genistein, Equol, O-Desmethylangolensin, Coumestrol, Matairesinol, Enterodiol, and Enterolactone

Phytoestrogens are plant compounds that can effect in vivo estrogen signaling. These "plant estrogens" have been shown to affect the human endocrine system and thus may affect human health. Consumption of foods rich in phytoestrogens is associated with many positive health outcomes: reduced risk for cancer and heart disease, reduction of menopausal symptoms and modulation of osteoporosis. The potential of these compounds to impact human health has led to the need for rapid, sensitive, and precise assays for phytoestrogen metabolites in physiological matrices. Previous methods for phytoestrogen quantitation used less selective and/or less sensitive techniques. Many laboratories use HPLC coupled with UV detection (1, 2) due to its low cost, although these methods lack selectivity and sensitivity. A novel approach using HPLC with coulometric array detection (3) shows promise for increased selectivity and sensitivity, but selectivity depends greatly on redox potential, resulting in inadequate sensitivity for some important phytoestrogens in complex matrices such as serum. The application of capillary electrophoresis-tandem mass spectrometry to the detection of phytoestrogens shows promise of improved sensitivity and specificity, but this technique has yet to be fully utilized due to difficulties in handling very small sample volumes. GC-MS methods provide needed selectivity and sensitivity for the analysis of phytoestrogens in biological matrices (4, 5) but require cumbersome derivatization steps for these nonvolatile compounds. HPLC-MS does not require derivatization, and therefore results in much higher throughput. Barnes et al. (6) developed an HPLC-MS/MS method for the detection of phytoestrogens in serum and urine; however, the method did not chromatographically resolve all of the major phytoestrogens and included only one internal standard.

Here we present an HPLC-MS/MS method that builds on the work by Barnes et al. (6) to result in a method with improved selectivity, sensitivity, and precision for the quantitative detection of phytoestrogens in human urine and serum. The method uses enzymatic deconjugation of the phytoestrogens followed by solid-phase extraction and reverse-phase HPLC to resolve the analytes. The phytoestrogens are detected using a Sciex API III heated nebulizer-atmospheric pressure chemical ionization (HN-APCI) interface coupled with MS/MS. This method allows for rapid detection of the major isoflavones and lignans in human serum and urine with limits of detection in the low ppb range. Selectivity of the method is insured by the combination of tandem mass spectrometry and chromatographic resolution. Internal standards for each of the analytes improved the precision of the assay.

Analytic Notes

Measures of urinary phthalates, urinary polyaromatic hydrocarbons, and urinary phytoestrogens are assessed in participants aged 6 years and over on a one-third sample. Use the special weights included in this data file when analyzing data. Read the "Special Sample Weights for this Dataset" information provided below before beginning analysis.

Mono-ethyl phthalate (URXMEP), Mono-benzyl phthalate (URXMZP), Mono-n-octyl phthalate (URXMOP), Mono-isononyl phthalate (URXMNP), and Mono-cyclohexyl phthalate (URXMCP):
NHANES recently learned that analytical standards used since 1999 and used for several metabolites were of insufficient purity. As a result of further investigation, a correction factor was applied to each value for the 5 metabolites.

Detection limits

For the seven phthalate metabolites and the six phytoestrogens, the detection limit was constant. In cases, where the result was below the limit of detection, the value for that variable is the detection limit divided by the square root of two. For the polyaromatic hydrocarbon analytes, the detection limit was variable. Two variables are provided for each of these analytes. The variable named URD_LC indicates whether the results was below the limit of detection. There are two values: "0" and "1"; "1" indicates that the result was below the limit of detection. The other variable named URX_ provides the analytic result for that analyte. In cases, where the result was below the limit of detection, the value for that variable is the detection limit divided by the square root of two.

Special Notes for this Dataset

The analysis of NHANES 1999–2000 laboratory data must be conducted with the key survey design and basic demographic variables. The NHANES 1999–2000 Household Questionnaire Data Files contain demographic data, health indicators, and other related information collected during household interviews. The Household Questionnaire and other data files may be linked to the laboratory data file using the unique survey participant identifier SEQN.

Special Sample Weights for this Dataset

Special sample weights are required to analyze these data properly. Measures of this urinary multi-analyte profile are assessed in participants aged 6 years and over on a randomly selected 1/3 subsample. Specific sample weights for this subsample are included in this data file and should be used when analyzing these data.

The dataset includes 2-year and 4-year subsample weights. The 4-year weights should be used if these 2001–2002 data are combined with 1999–2000 data. The 1999–2000 data files have been updated to include the subsample 4-year weights. The recommended procedure for variance estimation requires use of stratum and PSU variables (SDMVSTRA and SDMVPSU, respectively), which are included in the demographic data file for each data release. For further information, see the NHANES Analytic Guidelines, June 2004 version at: http://www.cdc.gov/nchs/data/nhanes/nhanes_general_guidelines_june_04.pdf.

 

References

Codebook and Frequencies

SEQN - Respondent sequence number

Variable Name:
SEQN
SAS Label:
Respondent sequence number
English Text:
Respondent sequence number.
Target:
Both males and females 6 YEARS - 150 YEARS

WTSPH4YR - Phthalate Subsample 4 Year Mec Weight

Variable Name:
WTSPH4YR
SAS Label:
Phthalate Subsample 4 Year Mec Weight
English Text:
Phthalate Subsample 4 year Mec
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 308716.27489 Range of Values 2648 2648
. Missing 0 2648

WTSPH2YR - Phthalate Subsample 2 Year Mec Weight

Variable Name:
WTSPH2YR
SAS Label:
Phthalate Subsample 2 Year Mec Weight
English Text:
Phthalate Subsample 2 year Mec Weight
English Instructions:
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 784084.01503 Range of Values 2648 2648
. Missing 0 2648

WTSPH01 - Phthalate Mec Weight Jack Knife Rep 01

Variable Name:
WTSPH01
SAS Label:
Phthalate Mec Weight Jack Knife Rep 01
English Text:
Phthalate Mec Weight Jack Knife Rep 01
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 765268.1804500 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH02 - Phthalate Mec Weight Jack Knife Rep 02

Variable Name:
WTSPH02
SAS Label:
Phthalate Mec Weight Jack Knife Rep 02
English Text:
Phthalate Mec Weight Jack Knife Rep 02
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 779596.2380300 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH03 - Phthalate Mec Weight Jack Knife Rep 03

Variable Name:
WTSPH03
SAS Label:
Phthalate Mec Weight Jack Knife Rep 03
English Text:
Phthalate Mec Weight Jack Knife Rep 03
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 784169.0941500 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH04 - Phthalate Mec Weight Jack Knife Rep 04

Variable Name:
WTSPH04
SAS Label:
Phthalate Mec Weight Jack Knife Rep 04
English Text:
Phthalate Mec Weight Jack Knife Rep 04
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 792866.4112900 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH05 - Phthalate Mec Weight Jack Knife Rep 05

Variable Name:
WTSPH05
SAS Label:
Phthalate Mec Weight Jack Knife Rep 05
English Text:
Phthalate Mec Weight Jack Knife Rep 05
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 780507.4001900 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH06 - Phthalate Mec Weight Jack Knife Rep 06

Variable Name:
WTSPH06
SAS Label:
Phthalate Mec Weight Jack Knife Rep 06
English Text:
Phthalate Mec Weight Jack Knife Rep 06
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 803223.9563400 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH07 - Phthalate Mec Weight Jack Knife Rep 07

Variable Name:
WTSPH07
SAS Label:
Phthalate Mec Weight Jack Knife Rep 07
English Text:
Phthalate Mec Weight Jack Knife Rep 07
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 768135.8023000 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH08 - Phthalate Mec Weight Jack Knife Rep 08

Variable Name:
WTSPH08
SAS Label:
Phthalate Mec Weight Jack Knife Rep 08
English Text:
Phthalate Mec Weight Jack Knife Rep 08
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 779055.0525600 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH09 - Phthalate Mec Weight Jack Knife Rep 09

Variable Name:
WTSPH09
SAS Label:
Phthalate Mec Weight Jack Knife Rep 09
English Text:
Phthalate Mec Weight Jack Knife Rep 09
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 794668.1316000 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH10 - Phthalate Mec Weight Jack Knife Rep 10

Variable Name:
WTSPH10
SAS Label:
Phthalate Mec Weight Jack Knife Rep 10
English Text:
Phthalate Mec Weight Jack Knife Rep 10
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 789159.7512100 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH11 - Phthalate Mec Weight Jack Knife Rep 11

Variable Name:
WTSPH11
SAS Label:
Phthalate Mec Weight Jack Knife Rep 11
English Text:
Phthalate Mec Weight Jack Knife Rep 11
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 784645.3988500 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH12 - Phthalate Mec Weight Jack Knife Rep 12

Variable Name:
WTSPH12
SAS Label:
Phthalate Mec Weight Jack Knife Rep 12
English Text:
Phthalate Mec Weight Jack Knife Rep 12
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 780525.4131700 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH13 - Phthalate Mec Weight Jack Knife Rep 13

Variable Name:
WTSPH13
SAS Label:
Phthalate Mec Weight Jack Knife Rep 13
English Text:
Phthalate Mec Weight Jack Knife Rep 13
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 774699.5958300 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH14 - Phthalate Mec Weight Jack Knife Rep 14

Variable Name:
WTSPH14
SAS Label:
Phthalate Mec Weight Jack Knife Rep 14
English Text:
Phthalate Mec Weight Jack Knife Rep 14
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 800794.0390600 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH15 - Phthalate Mec Weight Jack Knife Rep 15

Variable Name:
WTSPH15
SAS Label:
Phthalate Mec Weight Jack Knife Rep 15
English Text:
Phthalate Mec Weight Jack Knife Rep 15
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 800067.9192800 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH16 - Phthalate Mec Weight Jack Knife Rep 16

Variable Name:
WTSPH16
SAS Label:
Phthalate Mec Weight Jack Knife Rep 16
English Text:
Phthalate Mec Weight Jack Knife Rep 16
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 771559.1581200 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH17 - Phthalate Mec Weight Jack Knife Rep 17

Variable Name:
WTSPH17
SAS Label:
Phthalate Mec Weight Jack Knife Rep 17
English Text:
Phthalate Mec Weight Jack Knife Rep 17
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 770860.4819000 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH18 - Phthalate Mec Weight Jack Knife Rep 18

Variable Name:
WTSPH18
SAS Label:
Phthalate Mec Weight Jack Knife Rep 18
English Text:
Phthalate Mec Weight Jack Knife Rep 18
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 778875.0090200 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH19 - Phthalate Mec Weight Jack Knife Rep 19

Variable Name:
WTSPH19
SAS Label:
Phthalate Mec Weight Jack Knife Rep 19
English Text:
Phthalate Mec Weight Jack Knife Rep 19
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 806797.8028000 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH20 - Phthalate Mec Weight Jack Knife Rep 20

Variable Name:
WTSPH20
SAS Label:
Phthalate Mec Weight Jack Knife Rep 20
English Text:
Phthalate Mec Weight Jack Knife Rep 20
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 785428.9991600 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH21 - Phthalate Mec Weight Jack Knife Rep 21

Variable Name:
WTSPH21
SAS Label:
Phthalate Mec Weight Jack Knife Rep 21
English Text:
Phthalate Mec Weight Jack Knife Rep 21
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 790952.9526000 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH22 - Phthalate Mec Weight Jack Knife Rep 22

Variable Name:
WTSPH22
SAS Label:
Phthalate Mec Weight Jack Knife Rep 22
English Text:
Phthalate Mec Weight Jack Knife Rep 22
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 784232.9686100 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH23 - Phthalate Mec Weight Jack Knife Rep 23

Variable Name:
WTSPH23
SAS Label:
Phthalate Mec Weight Jack Knife Rep 23
English Text:
Phthalate Mec Weight Jack Knife Rep 23
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 777601.8066600 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH24 - Phthalate Mec Weight Jack Knife Rep 24

Variable Name:
WTSPH24
SAS Label:
Phthalate Mec Weight Jack Knife Rep 24
English Text:
Phthalate Mec Weight Jack Knife Rep 24
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 784675.4300300 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH25 - Phthalate Mec Weight Jack Knife Rep 25

Variable Name:
WTSPH25
SAS Label:
Phthalate Mec Weight Jack Knife Rep 25
English Text:
Phthalate Mec Weight Jack Knife Rep 25
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 784085.3124100 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH26 - Phthalate Mec Weight Jack Knife Rep 26

Variable Name:
WTSPH26
SAS Label:
Phthalate Mec Weight Jack Knife Rep 26
English Text:
Phthalate Mec Weight Jack Knife Rep 26
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 777322.1659800 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH27 - Phthalate Mec Weight Jack Knife Rep 27

Variable Name:
WTSPH27
SAS Label:
Phthalate Mec Weight Jack Knife Rep 27
English Text:
Phthalate Mec Weight Jack Knife Rep 27
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 795953.9385700 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH28 - Phthalate Mec Weight Jack Knife Rep 28

Variable Name:
WTSPH28
SAS Label:
Phthalate Mec Weight Jack Knife Rep 28
English Text:
Phthalate Mec Weight Jack Knife Rep 28
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 800311.1003200 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH29 - Phthalate Mec Weight Jack Knife Rep 29

Variable Name:
WTSPH29
SAS Label:
Phthalate Mec Weight Jack Knife Rep 29
English Text:
Phthalate Mec Weight Jack Knife Rep 29
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 789325.0018400 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH30 - Phthalate Mec Weight Jack Knife Rep 30

Variable Name:
WTSPH30
SAS Label:
Phthalate Mec Weight Jack Knife Rep 30
English Text:
Phthalate Mec Weight Jack Knife Rep 30
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 806226.4795700 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH31 - Phthalate Mec Weight Jack Knife Rep 31

Variable Name:
WTSPH31
SAS Label:
Phthalate Mec Weight Jack Knife Rep 31
English Text:
Phthalate Mec Weight Jack Knife Rep 31
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 788890.8281600 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH32 - Phthalate Mec Weight Jack Knife Rep 32

Variable Name:
WTSPH32
SAS Label:
Phthalate Mec Weight Jack Knife Rep 32
English Text:
Phthalate Mec Weight Jack Knife Rep 32
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 752108.9653900 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH33 - Phthalate Mec Weight Jack Knife Rep 33

Variable Name:
WTSPH33
SAS Label:
Phthalate Mec Weight Jack Knife Rep 33
English Text:
Phthalate Mec Weight Jack Knife Rep 33
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 778810.0049300 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH34 - Phthalate Mec Weight Jack Knife Rep 34

Variable Name:
WTSPH34
SAS Label:
Phthalate Mec Weight Jack Knife Rep 34
English Text:
Phthalate Mec Weight Jack Knife Rep 34
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 784645.2146500 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH35 - Phthalate Mec Weight Jack Knife Rep 35

Variable Name:
WTSPH35
SAS Label:
Phthalate Mec Weight Jack Knife Rep 35
English Text:
Phthalate Mec Weight Jack Knife Rep 35
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 742569.1556100 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH36 - Phthalate Mec Weight Jack Knife Rep 36

Variable Name:
WTSPH36
SAS Label:
Phthalate Mec Weight Jack Knife Rep 36
English Text:
Phthalate Mec Weight Jack Knife Rep 36
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 779052.7439200 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH37 - Phthalate Mec Weight Jack Knife Rep 37

Variable Name:
WTSPH37
SAS Label:
Phthalate Mec Weight Jack Knife Rep 37
English Text:
Phthalate Mec Weight Jack Knife Rep 37
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 785043.8441600 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH38 - Phthalate Mec Weight Jack Knife Rep 38

Variable Name:
WTSPH38
SAS Label:
Phthalate Mec Weight Jack Knife Rep 38
English Text:
Phthalate Mec Weight Jack Knife Rep 38
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 813853.1312800 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH39 - Phthalate Mec Weight Jack Knife Rep 39

Variable Name:
WTSPH39
SAS Label:
Phthalate Mec Weight Jack Knife Rep 39
English Text:
Phthalate Mec Weight Jack Knife Rep 39
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 787513.4157500 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH40 - Phthalate Mec Weight Jack Knife Rep 40

Variable Name:
WTSPH40
SAS Label:
Phthalate Mec Weight Jack Knife Rep 40
English Text:
Phthalate Mec Weight Jack Knife Rep 40
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 787966.0041400 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH41 - Phthalate Mec Weight Jack Knife Rep 41

Variable Name:
WTSPH41
SAS Label:
Phthalate Mec Weight Jack Knife Rep 41
English Text:
Phthalate Mec Weight Jack Knife Rep 41
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 811402.5606500 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH42 - Phthalate Mec Weight Jack Knife Rep 42

Variable Name:
WTSPH42
SAS Label:
Phthalate Mec Weight Jack Knife Rep 42
English Text:
Phthalate Mec Weight Jack Knife Rep 42
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 785193.4511400 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH43 - Phthalate Mec Weight Jack Knife Rep 43

Variable Name:
WTSPH43
SAS Label:
Phthalate Mec Weight Jack Knife Rep 43
English Text:
Phthalate Mec Weight Jack Knife Rep 43
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 789336.2903500 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH44 - Phthalate Mec Weight Jack Knife Rep 44

Variable Name:
WTSPH44
SAS Label:
Phthalate Mec Weight Jack Knife Rep 44
English Text:
Phthalate Mec Weight Jack Knife Rep 44
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 786131.3163400 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH45 - Phthalate Mec Weight Jack Knife Rep 45

Variable Name:
WTSPH45
SAS Label:
Phthalate Mec Weight Jack Knife Rep 45
English Text:
Phthalate Mec Weight Jack Knife Rep 45
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 717399.1618200 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH46 - Phthalate Mec Weight Jack Knife Rep 46

Variable Name:
WTSPH46
SAS Label:
Phthalate Mec Weight Jack Knife Rep 46
English Text:
Phthalate Mec Weight Jack Knife Rep 46
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 803502.1224200 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH47 - Phthalate Mec Weight Jack Knife Rep 47

Variable Name:
WTSPH47
SAS Label:
Phthalate Mec Weight Jack Knife Rep 47
English Text:
Phthalate Mec Weight Jack Knife Rep 47
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 748407.5819300 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH48 - Phthalate Mec Weight Jack Knife Rep 48

Variable Name:
WTSPH48
SAS Label:
Phthalate Mec Weight Jack Knife Rep 48
English Text:
Phthalate Mec Weight Jack Knife Rep 48
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 785023.7317800 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH49 - Phthalate Mec Weight Jack Knife Rep 49

Variable Name:
WTSPH49
SAS Label:
Phthalate Mec Weight Jack Knife Rep 49
English Text:
Phthalate Mec Weight Jack Knife Rep 49
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 780791.2082200 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH50 - Phthalate Mec Weight Jack Knife Rep 50

Variable Name:
WTSPH50
SAS Label:
Phthalate Mec Weight Jack Knife Rep 50
English Text:
Phthalate Mec Weight Jack Knife Rep 50
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 775462.4038700 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH51 - Phthalate Mec Weight Jack Knife Rep 51

Variable Name:
WTSPH51
SAS Label:
Phthalate Mec Weight Jack Knife Rep 51
English Text:
Phthalate Mec Weight Jack Knife Rep 51
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 771051.1322600 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

WTSPH52 - Phthalate Mec Weight Jack Knife Rep 52

Variable Name:
WTSPH52
SAS Label:
Phthalate Mec Weight Jack Knife Rep 52
English Text:
Phthalate Mec Weight Jack Knife Rep 52
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.0000000 to 783993.6852100 Range of Values 0 0
0 0 2648 2648
. Missing 0 2648

URXMBP - Mono-n-butyl phthalate (ng/mL)

Variable Name:
URXMBP
SAS Label:
Mono-n-butyl phthalate (ng/mL)
English Text:
Mono-n-butyl phthalate (ng/mL)
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.6 to 1192.9 Range of Values 2541 2541
. Missing 107 2648

URXMCP - Mono-cyclohexyl phthalate (ng/mL)

Variable Name:
URXMCP
SAS Label:
Mono-cyclohexyl phthalate (ng/mL)
English Text:
Mono-cyclohexyl phthalate (ng/mL)
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
1.2792 to 131.856 Range of Values 2541 2541
. Missing 107 2648

URXMEP - Mono-ethyl phthalate (ng/mL)

Variable Name:
URXMEP
SAS Label:
Mono-ethyl phthalate (ng/mL)
English Text:
Mono-ethyl phthalate (ng/mL)
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.858 to 26359.08 Range of Values 2536 2536
. Missing 112 2648

URXMHP - Mono-(2-ethyl)-hexyl phthalate (ng/mL)

Variable Name:
URXMHP
SAS Label:
Mono-(2-ethyl)-hexyl phthalate (ng/mL)
English Text:
Mono-(2-ethyl)-hexyl phthalate (ng/mL)
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.8 to 615.6 Range of Values 2541 2541
. Missing 107 2648

URXMNP - Mono-isononyl phthalate (ng/mL)

Variable Name:
URXMNP
SAS Label:
Mono-isononyl phthalate (ng/mL)
English Text:
Mono-isononyl phthalate (ng/mL)
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.8712 to 454.608 Range of Values 2541 2541
. Missing 107 2648

URXMOP - Mono-n-octyl phthalate (ng/mL)

Variable Name:
URXMOP
SAS Label:
Mono-n-octyl phthalate (ng/mL)
English Text:
Mono-n-octyl phthalate (ng/mL)
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
1.0691 to 63.336 Range of Values 2541 2541
. Missing 107 2648

URXMZP - Mono-benzyl phthalate (ng/mL)

Variable Name:
URXMZP
SAS Label:
Mono-benzyl phthalate (ng/mL)
English Text:
Mono-benzyl phthalate (ng/mL)
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.4073 to 1383.12 Range of Values 2541 2541
. Missing 107 2648

URXDAZ - Daidzein (ng/mL)

Variable Name:
URXDAZ
SAS Label:
Daidzein (ng/mL)
English Text:
Daidzein (ng/mL)
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.35 to 46000 Range of Values 2553 2553
. Missing 95 2648

URXDMA - o-Desmethylangolensin (O-DMA) (ng/mL)

Variable Name:
URXDMA
SAS Label:
o-Desmethylangolensin (O-DMA) (ng/mL)
English Text:
o-Desmethylangolensin (O-DMA) (ng/mL)
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.14 to 8500 Range of Values 2271 2271
. Missing 377 2648

URXEQU - Equol (ng/mL)

Variable Name:
URXEQU
SAS Label:
Equol (ng/mL)
English Text:
Equol (ng/mL)
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
2.12 to 3700 Range of Values 2182 2182
. Missing 466 2648

URXETD - Enterodiol (ng/mL)

Variable Name:
URXETD
SAS Label:
Enterodiol (ng/mL)
English Text:
Enterodiol (ng/mL)
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.57 to 18000 Range of Values 2527 2527
. Missing 121 2648

URXETL - Enterolactone (ng/mL)

Variable Name:
URXETL
SAS Label:
Enterolactone (ng/mL)
English Text:
Enterolactone (ng/mL)
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.42 to 22640 Range of Values 2548 2548
. Missing 100 2648

URXGNS - Genistein (ng/mL)

Variable Name:
URXGNS
SAS Label:
Genistein (ng/mL)
English Text:
Genistein (ng/mL)
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.21 to 24800 Range of Values 2557 2557
. Missing 91 2648

URXP09 - 3-fluoranthene (ng/L)

Variable Name:
URXP09
SAS Label:
3-fluoranthene (ng/L)
English Text:
3-fluoranthene (ng/L)
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
2.5 to 5489.5 Range of Values 2236 2236
. Missing 412 2648

URDP09LC - 3-fluoranthene comment code

Variable Name:
URDP09LC
SAS Label:
3-fluoranthene comment code
English Text:
3-fluoranthene comment code
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 Detectable results 1600 1600
1 Below detectable limit 636 2236
. Missing 412 2648

URXUCR - Creatinine, urine (mg/dL)

Variable Name:
URXUCR
SAS Label:
Creatinine, urine (mg/dL)
English Text:
Creatinine, urine (mg/dL)
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
2 to 650 Range of Values 2575 2575
. Missing 73 2648