Table of Contents

Locator Record

Title: Urinary Creatinine and Albumin (LAB16 )
Contact Number: 1-866-441-NCHS
Years of Content: 1999 - 2000
First Published: June, 2002
Revised: NA
Access Constraints: None
Use Constraints: None
Geographic Coverage: National
Subject:Urinary Creatinine and Albumin
Record Source: NHANES 1999 - 2000
Survey Methodology: NHANES 1999 - 2000 is a stratified multistage probability sample of the civilian non-institutionalized population of the U.S.
Medium: NHANES Web site; SAS transport files

Component Description

Urinary albumin and creatinine are measured. Related survey questionnaire data include information on analgesic product use and incontinence.

Eligible Sample

Participants aged 6 years and older

Description of Laboratory Methodology

 Data Collection Methods

A casual urine specimen is collected from the participant. In the laboratory, the urine specimen is processed, stored, and shipped to the University of Minnesota for analysis.

Examination Protocol

Detailed specimen collection and processing instructions are discussed in the NHANES Laboratory/Medical Technologists Procedures Manual (LPM). Vials were stored under appropriate frozen (-20 degrees Centigrade) conditions until they were shipped to University of Minnesota for testing. The analytical methods are described in the Analytic methodology section.

Analytic Methodology

Urinary Creatinine

Creatinine analysis uses a Jaffé rate reaction, in which creatinine reacts with picrate in an alkaline solution to form a red creatinine-picrate complex. The reaction is measured with a CX3 analyzer. The rate of the color development is measured 25.6 sec after sample injection at 520 nm and at 560 nm. The rate difference between the two wavelengths is proportional to the concentration of creatinine in the reaction cup. The procedures described below are the standard protocols of the Fairview University Medical Center (FUMC)1,2,3,4,5.

Creatinine, the waste product derived from creatine, is released into the plasma at a relatively constant rate. The amount of creatinine per unit of muscle mass is constant; therefore, creatinine is the best indicator of impaired kidney function.

Urinary Albumin

A solid-phase fluorescent immunoassay for the measurement of human urinary albumin is described by Chavers et al.6. The fluorescent immunoassay is a non-competitive, double-antibody method for the determination of human albumin in
urine. Antibody to human albumin is covalently attached to derivatized polyacrylamide beads. The solid-phase antibody is reacted with a urine specimen. The urine albumin-antigen complexes with the solid-phase antibody. This complex then reacts with fluorescein-labeled antibody. Remove the unattached fluorescent antibody by washing during centrifugation. Determine the fluorescence of the stable solid-phase antibody complex with a fluorometer. The fluorescence is directly proportional to the amount of urine albumin present. The standard curve is 0.5-20 μg/mL albumin.

Increased microalbuminuria is a sign of renal disease and may be predictive of nephropathy risk in patients with insulin-dependent diabetes. Results of the fluorescent immunoassay (FIA) are reproducible, and the test is accurate and sensitive for the detection of human urinary albumin excretion. It is especially useful for measurement of low levels of urinary albumin not detectable by dipstick methods. The FIA assay resembles the radio-immunoassay (RIA) in technique and sensitivity without the potential health hazards associated with the handling of isotopes in the laboratory6.

References

Codebook and Frequencies

SEQN - Respondent sequence number

Variable Name:
SEQN
SAS Label:
Respondent sequence number
English Text:
Respondent sequence number.
Target:
Both males and females 6 YEARS - 150 YEARS

URXUMA - Albumin, urine (ug/mL)

Variable Name:
URXUMA
SAS Label:
Albumin, urine (ug/mL)
English Text:
Albumin, urine (ug/mL)
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.2 to 16500 Range of Values 7606 7606
. Missing 213 7819

URXUMASI - Albumin, urine (mg/L) SI

Variable Name:
URXUMASI
SAS Label:
Albumin, urine (mg/L) SI
English Text:
Albumin, urine (mg/L) SI
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.2 to 16500 Range of Values 7606 7606
. Missing 213 7819

URXUCR - Creatinine, urine (mg/dL)

Variable Name:
URXUCR
SAS Label:
Creatinine, urine (mg/dL)
English Text:
Creatinine, urine (mg/dL)
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
2 to 650 Range of Values 7606 7606
. Missing 213 7819

URXUCRSI - Creatinine, urine (umol/L)

Variable Name:
URXUCRSI
SAS Label:
Creatinine, urine (umol/L)
English Text:
Creatinine, urine (umol/L)
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
177 to 57460 Range of Values 7606 7606
. Missing 213 7819