Table of Contents

Locator Record

Title: Urinary Chlamydia and Gonorrhea (L05RDC_A )
Contact Number: 1-866-441-NCHS
Years of Content: 1999 - 2000
First Published: June, 2009
Revised: NA
Access Constraints: RDC Only
Use Constraints: RDC Only
Geographic Coverage: National
Subject:Chlamydia and Gonorrhea
Record Source: NHANES 1999 - 2000
Survey Methodology: NHANES 1999 - 2000 is a stratified multistage probability sample of the civilian non-institutionalized population of the U.S.
Medium: NHANES Web site; SAS transport files

Component Description

Urinary chlamydia and Urinary Gonorrhea

Sexually transmitted infections caused by Chlamydia trachomatis and Neisseria
gonorrhoeae may lead to pelvic inflammatory disease, ectopic pregnancy, infertility, and
chronic pelvic pain in women. They are also associated with increased risk of HIV
transmission. Pregnant women may transmit infection to their newborn causing serious
medical complications. At present there are no reliable estimates on the prevalence of
chlamydial and gonococcal infections in the general population of the United States.

NHANES offers an opportunity to assess the prevalence of chlamydial and gonococcal
infection in the general population and to monitor trends in prevalence as prevention
programs are established and expanded.

Eligible Sample

Participants aged 14 to 39 years are tested. Public data file includes data for persons
18-39 years of age. Please see notes about the availability of data for adolescents 14-
17 years of age.

Description of Laboratory Methodology

Data Collection Methods

Urine specimens are processed, stored, and shipped to the National Centers for
Infectious Diseases for testing.

Examination Protocol

Detailed specimen collection and processing instructions are discussed in the NHANES
Laboratory/Medical Technologists Procedures Manual (LPM). Vials were stored under
appropriate frozen (minus 20 degrees Centigrade) conditions until they were shipped to
the National Center for Infectious Diseases for testing.

Analytic Methodology

Urinary chlamydia

The Chlamydia trachomatis assay uses LCR TM (l igase chain reaction) amplification
technology in the LCx Probe System for the direct, qualitative detection of plasmid DNA
of Chlamydia trachomatis.

The LCx Chlamydia trachomatis assay uses the nucleic acid amplication method LCR
to detect the presence of C. trachomatis plasmid DNA directly in clinical specimens.
The four oligonucleotide probes in the LCx assay recognize and hybridize to a specific
target sequence within the C. trachomatis plasmid DNA. The oligonuleotides are
designed to be complementary to the target sequence so that in the presence of target,
the probes will bind adjacent to one another. They can then be enzymatically joined to
form the amplification product, which subsequently serves as an additional target
sequence during further rounds of amplication. The product of the LCR reaction is
detected on the Abbott LCx analyzer.

Urinary gonorrhea

The Neisseria gonorrhoeae assay uses LCR TM (ligase chain reaction) amplification
technology in the LCx Probe System for the direct, qualitative detection of a specific
target nucleic acid sequence in the Opa gene of Neisseria gonorrhoeae.

The LCx Neisseria gonorrhoeae assay uses the nucleic acid amplication method LCR to
detect the presence of Neisseria gonorrhoeae. The four oligonucleotide probes in the
LCx assay recognize and hybridize to a specific target sequence within the Opa gene of
Neisseria gonorrhoeae DNA. The oligonuleotides are designed to be complementary to
the target sequence so that in the presence of target, the probes will bind adjacent to
one another. They can then be enzymatically joined to form the amplification product
which subsequently serves as an additional target sequence during further rounds of
amplication. The product of the LCR reaction is detected on the Abbott LCx analyzer.

Analytic Notes

Urinary chlamydia and gonorrhea data for youth 14-17 years of age will be available in
the NCHS Research Data Center (RDC).

References

Codebook and Frequencies

SEQN - Respondent sequence number

Variable Name:
SEQN
SAS Label:
Respondent sequence number
English Text:
Respondent sequence number.
Target:
Both males and females 14 YEARS - 17 YEARS

URXUCL - Chlamydia, urine

Variable Name:
URXUCL
SAS Label:
Chlamydia, urine
English Text:
Chlamydia, urine
Target:
Both males and females 14 YEARS - 17 YEARS
Code or Value Value Description Count Cumulative Skip to Item
1 Positive 47 47
2 Negative 1056 1103
3 Indeterminate 0 1103
. Missing 38 1141

URXUGC - Gonorrhea, urine

Variable Name:
URXUGC
SAS Label:
Gonorrhea, urine
English Text:
Gonorrhea, urine
Target:
Both males and females 14 YEARS - 17 YEARS
Code or Value Value Description Count Cumulative Skip to Item
1 Positive 12 12
2 Negative 1091 1103
3 Indeterminate 0 1103
. Missing 38 1141