Table 1. Summary of ELISA Result
Serum Source Antibody Detected Results
None None Human IgE Blank
Cry9c Pre-1996 CDC controls Human IgE All nonreactive
Cry9c Atopic CDC controls Human IgE All nonreactive
Cry9c Epi-Aid cases Human IgE All nonreactive
Grass Positive controls* Human IgE All reactive to strongly reactive
Cat Positive controls* Human IgE All reactive to strongly reactive
Peanut Positive controls* Human IgE All reactive to strongly reactive
None None Goat IgG Blank
Cry9c Normal goat serum Goat IgG Nonreactive
Cry9c Hyperimmune goat serum** Goat IgG Strongly reactive
Nonreactive: Less than twofold blank reading
Reactive: Twofold to tenfold blank reading
Strongly reactive: Greater than tenfold blank reading
* Positive controls were obtained from IBT Laboratories, Inc.
** Hyperimmune goat serum was obtained from goats immunized with Cry9c.
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