Procedures for Collecting and Processing Environmental Specimens for Legionella spp.
- Collect water (1-liter samples, if possible) in sterile, screw-top bottles.
- Collect culture swabs of internal surfaces of faucets, aerators, and shower heads in a sterile, screw-top container (e.g., 50 mL plastic centrifuge tube). Submerge each swab in approximately 5 mL of sample water taken from the same device from which the sample was obtained.
- Transport samples and process in a laboratory proficient at culturing water specimens for Legionella spp., as soon as possible after collection.
- Test samples for the presence of Legionella spp. by using semiselective culture media using procedures specific to the cultivation and detection of Legionella spp.
- Barbaree JM, Gorman GW, Martin, WT, Fields, BS, Morrill WE. Protocol for sampling environmental sites for legionellae[922 KB, 5 pages]. Appl Environ Microbiol. 1987;53:1454-8.
- CDC. Procedures for the recovery of Legionella from the environment. Atlanta GA : US Department of Health and Human Services, Public Health Service. 1992;1-13.
Images and logos on this website which are trademarked/copyrighted or used with permission of the trademark/copyright or logo holder are not in the public domain. These images and logos have been licensed for or used with permission in the materials provided on this website. The materials in the form presented on this website may be used without seeking further permission. Any other use of trademarked/copyrighted images or logos requires permission from the trademark/copyright holder...more
This graphic notice means that you are leaving an HHS Web site. For more information, please see the Exit Notification and Disclaimer policy.