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The Methods of Antimicrobial Susceptibility Testing Educational Resource (MASTER) eLearning Curriculum

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The Methods of Antimicrobial Susceptibility Testing Educational Resource (MASTER) eLearning curriculum provides online training in the area of antimicrobial susceptibility testing for bacteria isolated from clinical specimens. 

Five courses available in the AST curriculum are:


MASTER: Antimicrobial Susceptibility Testing Methods

This course provides a detailed overview of AST methods including reference methods such as those described by the Clinical and Laboratory Standards Institute (CLSI) and commercial test systems.

MASTER: Antimicrobial Susceptibility Quality Assurance and Quality

This course provides a detailed overview of the components of quality control (QC) and quality assurance (QA) necessary for antimicrobial susceptibility testing.  

MASTER: Antimicrobial Susceptibility Gram Positive Organisms (coming soon)

(coming soon)

MASTER: Antimicrobial Susceptibility CLSI Standards

This eLearning course is designed to familiarize laboratorians with routine microscopy procedures used in the microbiology laboratory.

MASTER: Antimicrobial Susceptibility Gram Negative Organisms

(coming soon)

Audience: Laboratory professionals who have knowledge of basic medical microbiology and who perform antimicrobial susceptibility testing or are considering adding it to the laboratory’s testing menu.

Objectives: At the conclusion of this curriculum, the participant will be able to:

  • Associate disk diffusion, MIC, and commercial AST systems as antimicrobial susceptibility testing methods.
  • Recognize the role of CLSI in developing recommendations for antimicrobial susceptibility testing of bacteria isolated from clinical specimens.
  • Identify the components of a quality assurance and quality control program for antimicrobial susceptibility testing.
  • Review Gram positive organisms, Staphylococcus spp., Enterococcus spp., Streptococcus spp., and Anaerobes for AST.
  • Review Gram negative organisms, Enterobacteriaceae, Non-Enterobacteriaceae, and Haemophilus for AST.

Continuing Education: The Centers for Disease Control and Prevention Laboratory Training Branch is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E.® Program. These courses are approved for various contact hours.

View an updated list of CDC Laboratory Training courses that are available now

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  • Page last reviewed: February 10, 2014
  • Page last updated: March 20, 2014 The U.S. Government's Official Web PortalDepartment of Health and Human Services
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