Ceftriaxone-Resistant Salmonella enterica Serotype Newport, France
Svetlana Egorova,*1 Mohammed Timinouni,*2 Marie Demartin,* Sophie A. Granier,† Jean M. Whichard,‡ Vartul Sangal,§ Laëtitia Fabre,* Aurélia Delauné,* Maria Pardos,* Yves Millemann,¶ Emmanuelle Espié,# Mark Achtman,§** Patrick A.D. Grimont,* and François-Xavier Weill*
*Institut Pasteur, Paris, France; †Agence Française de
Sécurité Sanitaire des Aliments, Maisons-Alfort, France; ‡Centers for Disease
Control and Prevention, Atlanta, Georgia, USA; §Max-Planck-Institute für
Infektionsbiologie, Berlin, Germany; ¶Ecole Nationale Vétérinaire d’Alfort,
Maisons-Alfort; #Institut de Veille Sanitaire, Saint-Maurice, France; and
**University College, Cork, Ireland
Vol. 14, No. 6 • June 2008
| Appendix Table. Characteristics of Samonella spp. isolates used in this study* | ||||||||||
| Isolate | Date of isolation | Area of isolation† | Patient age group‡, sex | Source | Antimicrobial drug resistance phenotype§ | Class 1 integron size, kb (gene cassette) |
PFGE profile¶ | MLST type | Plasmid profile# | Plasmid restriction profile** |
| S. Newport isolates | ||||||||||
| 00-3525 | 2000 Jun | 75 | III-F | Stool | AFoxCazSSuCTe | _ | New6b (JJPX01.0014) | ST45 | 100, 50, 3.5 | R4 |
| 00-3767 | 2000 Jun | 60 | III-F | Stool | AFoxCazSSuCTe | _ | New2 | ST45 | >125, 3.5 | R3 |
| 00-3784 | 2000 Jun | 75 | II-M | Stool | AFoxCazSSpKToGSuCTe | 1 (aadA24) | New3 | ST45 | >125, 7 | R1 |
| 00-4165 | 2000 Jul | 62 | II-M | Stool | AFoxCazSSpKToGSuCTe | 1 (aadA24) | New 3 | ST45 | >125, 7 | R5 |
| 00-4652 | 2000 Jul | 60 | II-F | Stool | AFoxCazSSuCTe | _ | New 4 | ND | >125, 3.5 | R4 |
| 00-5089 | 2000 Jul | 2A | II-F | Stool | AFoxCazSSpKToGSuCTe | 1 (aadA24) | New 3 | ST45 | >125, 7 | R1 |
| 00-6399 | 2000 Sep | 92 | V-M | NK | AFoxCazSSuCTe | _ | New5 (JJPX01.0249) | ND | >125, 3.5 | R1 |
| 00-7093 | 2000 Sep | 77 | II-F | Stool | AFoxCazSSuCTe | _ | New 7 | ST45 | >125, 90 | R1 |
| 00-7098 | 2000 Sep | 93 | II-F | Stool | AFoxCazSSuCTe | _ | New6b (JJPX01.0014) | ND | >125, 90 | R1 |
| 00-7298 | 2000 Sep | 77 | IV-F | Stool | AFoxCazSSuCTe | _ | New6b (JJPX01.0014) | ND | >125, 90 | R1 |
| 00-7325 | 2000 Sep | 75 | IV-F | Stool | AFoxCazSSuCTe | _ | New6b (JJPX01.0014) | ST45 | >125, 90 | R1 |
| 00-7400 | 2000 Sep | 77 | IV-M | Stool | AFoxCazSSuCTe | _ | New6b (JJPX01.0014) | ND | >125 | R1 |
| 00-7490 | 2000 Sep | 89 | II-M | Stool | AFoxCazSSuCTe | _ | New6b (JJPX01.0014) | ND | >125, 90 | R1 |
| 00-7777 | 2000 Oct | 77 | II-F | NK | AFoxCazSSuCTe | _ | New6b (JJPX01.0014) | ND | >125, 90 | R1 |
| 00-8066 | 2000 Oct | 93 | II-F | Stool | AFoxCazSSuCTe | _ | New6b (JJPX01.0014) | ND | >125, 90 | R1 |
| 01-2010 | 2001 Apr | 92 | V-F | Stool | AFoxCazSSuCTe | _ | New6a (JJPX01.0176) | ND | >125, 60, 3.5 | R2 |
| 01-2288 | 2001 Arp | 93 | IV-F | Blood | AFoxCazSSuCTe | _ | New6a (JJPX01.0176) | ST45 | >125, 60, 3.5 | ND |
| 01-9637 | 2001 Dec | 95 | NK-F | Stool | AFoxCazSSuCTe | _ | New6b (JJPX01.0014) | ND | >125, 3.5 | R1 |
| 01-9867 | 2001 Dec | 93 | III-F | Stool | AFoxCazSSuCTe | _ | New6b (JJPX01.0014) | ND | >125, 3.5 | ND |
| 01-10075 | 2001 Dec | 77 | II-M | Stool | AFoxCazSSuCTe | _ | New6b (JJPX01.0014) | ND | >125, 3.5 | ND |
| 02-7891 | 2002 Oct | 78 | III-M | Stool | AFoxCazSSuCTe | _ | New8a | ST45 | >125, 3.5 | R1 |
| 03-3125 | 2003 May | 62 | II-F | Stool | AFoxCazSSuCTe | _ | New8b | ND | >125, 70, 3.5 | R2 |
| 03-3136 | 2003 May | 62 | III-F | Stool | AFoxCazSSuCTe | _ | New8a | ND | >125, 3.5 | R1 |
| 03-3179 | 2003 May | 62 | III-M | Stool | AFoxCazSSuCTe | _ | New8c | ND | >125, 60 | R1 |
| 03-3184 | 2003 May | 62 | IV-F | Stool | AFoxCazSSuCTe | _ | New8a | ST45 | >125, 3.5 | R2 |
| 03-3222 | 2003 May | 62 | V-F | Blood | AFoxCazSSuCTe | _ | New8a | ND | >125, 3.5 | ND |
| 03-3224 | 2003 May | 59 | IV-M | Stool | AFoxCazSSuCTe | _ | New8a | ND | >125, 3.5 | ND |
| 03-3225 | 2003 May | 59 | IV-F | Stool | AFoxCazSSuCTe | _ | New8a | ND | >125, 3.5 | R1 |
| 03-3243 | 2003 May | 91 | III-M | Stool | AFoxCazSSuCTe | _ | New8a | ND | >125, 3.5 | ND |
| 03-3265 | 2003 May | 62 | II-M | Stool | AFoxCazSSuCTe | _ | New8d | ND | >125, 100, 60, 3.5 | ND |
| 03-3349 | 2003 May | 62 | NK-F | Stool | AFoxCazSSuCTe | _ | New8d | ST45 | >125, 100, 60, 3.5 | R2 |
| 03-3350 | 2003 May | 62 | II-M | Stool | AFoxCazSSuCTe | _ | New8c | ND | >125, 60, 3.5 | R1 |
| 03-3465 | 2003 May | 59 | V-M | Stool | AFoxCazSSuCTe | _ | New8a | ND | >125, 3.5 | ND |
| 03-3519 | 2003 May | 59 | II-F | Stool | AFoxCazSSuCTe | _ | New8d | ND | >125, 100, 60, 3.5 | R1 |
| 03-3603 | 2003 Jun | 59 | III-M | Stool | AFoxCazSSuCTe | _ | New8d | ND | >125, 100, 60, 3.5 | R1 |
| 03-3642 | 2003 Jun | 92 | IV-NK | Stool | AFoxCazSSuCTe | _ | New8a | ND | >125, 3.5 | ND |
| 03-4620 | 2003 Jul | 62 | IV-M | Stool | AFoxCazSSuCTe | _ | New8a | ND | >125, 3.5 | R1 |
| 03-5145 | 2003 Jul | 59 | III-M | Stool | AFoxCazSSuCTe | _ | New8d | ST45 | >125, 100, 60, 3.5 | R1 |
| 03-6521 | 2003 Sep | 59 | IV-M | Stool | AFoxCazSSuCTe | _ | New6d/8e | ST45 | >125, 100, 3.5 | R2 |
| 03-6773 | 2003 Sep | 62 | IV-F | Stool | AFoxCazSSuCTe | _ | New6d/8e | ND | >125, 100, 3.5 | R1 |
| 03-7268 | 2003 Sep | 62 | IV-M | Stool | AFoxCazSSuCTe | _ | New6b (JJPX01.0014) | ND | >125, 100, 3.5 | R1 |
| 03-7338 | 2003 Sep | 62 | IV-M | Stool | AFoxCazSSuCTe | _ | New6d/8e | ST45 | >125, 100, 3.5 | R1 |
| 03-8748 | 2003 Nov | 11 | V-M | Stool | ACroSuTmp | ND | New9 | ST118 | ND | ND |
| 03-9969 | 2003 Dec | 59 | V-F | Stool | AFoxCazSSuTe | _ | New6c | ST45 | >125, 40, 5 | R6 |
| 04-4556 | 2004 Jul | 11 | V-F | Stool | AFoxCazSSuCTe | _ | New9 | ND | >125, 3.5 | R1 |
| 04-9597 | 2004 Dec | 95 | V-F | Urine | AFoxCazSSuCTe | _ | New10 | ST45 | >125, 3.5 | R1 |
| S. Newport reference strain | ||||||||||
| 50 K | Pan susceptible | ND | New1 | ST31 | ND | ND | ||||
| CMY-2–producing S. Typhimurium isolate | ||||||||||
| 03-9243 | 2003 Oct | 44 | V-F | Stool | AFoxCazSKToGSuTmpCNal | – | STM53 | ND | 220, 90 | R7 |
| CMY-2–producing S. Agona isolates | ||||||||||
| 02-2049 | 2002 Apr | 93 | V-NK | Stool | AFoxCazSKSuTmpCTe | 1.2 (dfrA1-orfX) | Ago1 (JABX01.0055) | ND | 140 | R8 |
| 02-2059 | 2002 Apr | 77 | V-F | Stool | AFoxCazSKSuTmpCTe | 1.2(dfrA1-orfX) | Ago1 (JABX01.0055) | ND | 140 | R8 |
*PFGE, pulsed-field gel electrophoresis; MLST, multilocus sequence typing; ST, sequence type; ND, not determined; NK, not known. |
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| †Numbers are those of Départements (French administrative subdivisions) in the Paris metropolitan area (Départements 60, 75, 77, 78, and 91–95) and northern France (Départements 59 and 62). | ||||||||||
| ‡I, <1 y; II, 1–5 y; III, 6–14 y; IV, 15–64-y; V, >65 y. | ||||||||||
| §A, amoxicillin; Fox, cefoxitin; Caz, ceftazidime; S, streptomycin; Su, sulfonamides; C, chloramphenicol; Te, tetracycline; K, kanamycin; To, tobramycin; G, gentamicin; Sp, spectinomycin; Cro, ceftriaxone; Tmp, trimethoprim; Nal, nalidixic acid. | ||||||||||
| ¶Profiles in parentheses were obtained from the PulseNet USA database (www.cdc.gov/pulsenet). | ||||||||||
| #Determined by the alkaline lysis method. | ||||||||||
| **Profiles are shown in Figure 2. | ||||||||||
1Current affiliation: Institut Pasteur, St. Petersburg, Russia.
2Current affiliation: Institut Pasteur, Casablanca, Morocco.
This page posted May 23, 2008
