Laboratory Testing for Cholera
Rapid Diagnostic Testing
WHO Identification and Testing of Bacterial Pathogens in the Developing World
CDC Méthodes de laboratoire pour le diagnostic de la dysenterie épidémique et du choléra1
- couverture, l’introduction et la table des matières [PDF – 8 pages]
- Chapitre 1 – Rôle des laboratoires cliniques en santé publique [PDF – 8 pages]
- Chapitre 2 – Prélèvement et transport des échantillons dematières fécales [PDF – 6 pages]
- Chapitre 5 – Étiologie et épidémiologie du choléra [PDF – 5 pages]
- Chapitre 6 – Isolement et identification de V. cholerae O1 et O139 [PDF – 14 pages]
- Chapitre 9 – Sensibilité aux agents antimicrobiens (méthode de diffusion en milieu gélosé) [PDF – 14 pages]
- Chapitre 10 – Conservation des isolements [PDF – 2 pages]
- Chapitre 11 – Contrôle de qualité des milieux et réactifs [PDF – 4 pages]
- Chapitre 12 – Pratiques de sécurité standard dans le laboratoire de microbiologie [PDF – 6 pages]
- Chapitre 13 – Emballage et expédition d’échantillons cliniques ou d’agents étiologiques [PDF – 6 pages]
- Annexe A [PDF – 2 pages]
- Annexe B [PDF – 2 pages]
- Annexe C [PDF – 4 pages]
- Annexe D [PDF – 2 pages]
- Annexe E [PDF – 2 pages]
- Annexe F [PDF – 1 page]
- Annexe G [PDF – 1 page]
- Annexe H [PDF – 2 pages]
Clinical Laboratory Standards Institute documents
CDC Laboratory Methods for the Diagnosis of Vibrio cholerae2
- Chapter 4 – Isolation of Vibrio cholerae From Fecal Specimens
- Chapter 5 – Examination of Food and Environmental Samples
- Chapter 6 – Laboratory Identification of Vibrio cholerae
- Chapter 7 – Detection of Cholera Toxin
CDC Job Aids for Specimen Collection and Transport
- How to Collect a Fecal Specimen and Transfer to Transport Medium
Other Resources
- India. National Institute of Cholera and Enteric Diseases (NICED). National Institute of Cholera and Enteric Diseases.
Remarks
- This manual is to be used in countries with minimal laboratory capacity and few resources. It focuses on the epidemiology of cholera and the laboratory methods to isolate and identify Vibrio cholerae serogroups O1 and O139 and how to test their susceptibility to antimicrobial agents in the epidemic setting. The laboratory techniques and study methodology described provide accurate and useful information for the control of epidemics using a minimum of resources. Often, the countries that face the challenge of responding to an epidemic are those with the least resources. Therefore, the microbiology laboratory must use its resources wisely in order to have the greatest impact on reducing morbidity and mortality during an epidemic.
- This manual is intended for use by Reference Laboratories.