Adaora Adimora, M.D., M.P.H. Division of Infectious Diseases
University of North Carolina
Linda Alexander, Ph.D. President, American Social Health Association
Rhoda L. Ashley, Ph.D. Professor, Laboratory Medicine
University of Washington
Karl Beutner, M.D., Ph.D. Associate Clinical Professor of Dermatology
University of California, San Francisco
Gail Bolan , M.D., M.P.H. Director, STD Control Program
California Department of Health Services
Zane A. Brown, M.D. Professor of Perinatal Medicine,
Obstetrics and Gynecology
University of Washington
Jim Buehler, M.D. Perinatal Epidemiology
Georgia Department of Health
Ward Cates, M.D., M.P.H. President, Family Health International
Lawrence Corey, M.D. Professor of Laboratory Medicine and Medicine
University of Washington
Head, Program on Infectious Diseases
Fred Hutchinson Cancer Research Center
John Douglas, M.D. Associate Director,
Disease Control Service
Denver Public Health
Associate Professor of Medicine
University of Colorado
Charles Ebel Managing Editor
Sexual Health Magazine
Martin Fishbein, Ph.D. Annenburg School for Communication
Dennis Fortenberry, M.D. Department of Pediatrics
Indiana University
Geoff Garnett, Ph.D. Wellcome Trust Center for the Epidemiology
of Infectious Disease
Department of Zoology, University of Oxford
H. Hunter Handsfield, M.D. Professor of Medicine
University of Washington
Director, STD Control Program
Seattle-King County Department of Public Health
Penelope Hitchcock , D.V.M. Chief, STD Branch
National Institutes of Allergy & Infectious Diseases
National Institutes of Health
Edward Hook, III, M.D. Professor of Medicine/Infectious Diseases
University of Alabama at Birmingham
David B. Johnson STD/HIV Section
Georgia Department of Health
David W. Kimberlin, M.D. Assistant Professor of Pediatrics
University of Alabama at Birmingham
Andre Nahmias, M.D. Professor of Pediatrics
Emory University School of Medicine
Anne O'Leary, Ph.D. Associate Professor
Department of Psychology
Busch Campus
Rutgers University
Gary A. Richwald, M.D. Director, Los Angeles County
STD Program
Michael Ross, Ph.D. Associate Professor
University of Texas School of Public Health
Bradley Stoner, M.D., Ph.D. Assistant Professor of Medicine and Anthropology
Washington University Medical School
Anna Wald,, M.D., M.P.H. Assistant Professor of Medicine
University of Washington
Cheryl Walker, M.D. Associate Professor of Obstetrics and Gynecology
University of California at Irvine
Sevgi O. Aral, Ph.D. Associate Director for Science
Division of STD Prevention
National Center for HIV, STD, and TB Prevention
M. Blake Caldwell, M.D., M.P.H. Managed Care Activity
Office of Program Planning and Evaluation
Office of the Director
Susan DeLisle, A.R.N.P., M.P.H. Chief, Program Development and Support Branch
Division of STD Prevention
National Center for HIV, STD, and TB Prevention
Judith M. Graber, M.S. Viral Exanthems and Herpesvirus Branch
Division of Viral and Rickettsial Diseases
National Center for Infectious Diseases
Anne Haddix, Ph.D. Chief, Prevention Effectiveness Branch
Division of Prevention Research and Analytical
Methods
Epidemiology Program Office
Robert E. Johnson, M.D., M.P.H. Epidemiology and Surveillance Branch
Division of STD Prevention
National Center for HIV, STD, and TB Prevention
Mary L. Kamb, M.D., M.P.H. Prevention Services Research Branch
Division of HIV/AIDS Prevention-Surveillance
and Epidemiology
National Center for HIV, STD, and TB Prevention
William Kassler, M.D., M.P.H. Chief, Health Services Research and Evaluation
Branch
National Center for HIV, STD, and TB Prevention
Emily H. Koumans, M.D., M.P.H. Epidemiology and Surveillance Branch
Division of STD Prevention
National Center for HIV, STD, and TB Prevention
William C. Levine, M.D., M.Sc. Chief, Surveillance and Special Studies Section
Epidemiology and Surveillance Branch
Division of STD Prevention
National Center for HIV, STD, and TB Prevention
Mary McFarlane, Ph.D. Behavioral Interventions and Research Branch
Division of STD Prevention
National Center for HIV, STD, and TB Prevention
Philip E. Pellett, Ph.D. Chief, Herpes Virus Section
Viral Exanthems and Herpesvirus Branch
Division of Viral and Rickettsial Diseases
National Center for Infectious Diseases
Michele Reyes, Ph.D. Viral Exanthems and Herpesvirus Branch
Division of Viral and Rickettsial Diseases
National Center for Infectious Diseases
Phil Rhodes, Ph.D. Statistics and Data Management Branch
Division of STD Prevention
National Center for HIV, STD, and TB Prevention
D. Scott Schmid, Ph.D. Chief, Viral Immunology Section
Viral Exanthems and Herpesvirus Branch
Division of Viral and Rickettsial Diseases
National Center for Infectious Diseases
Jack N. Spencer Deputy Director
Division of STD Prevention
National Center for HIV, STD, and TB Prevention
Katherine M. Stone, M.D. Assistant Chief for Science
Epidemiology and Surveillance Branch
Division of STD Prevention
National Center for HIV, STD, and TB Prevention
Janet St. Lawrence, Ph.D. Chief, Behavioral Interventions and Research Branch
Division of STD Prevention
National Center for HIV, STD, and TB Prevention
Michael E. St. Louis, M.D. Chief, Epidemiology and Surveillance Branch
Division of STD PreventionNational
Center for HIV, STD, and TB Prevention
Guoyu Tao, Ph.D. Health Services Research and Evaluation Branch
Division of STD Prevention
National Center for HIV, STD, and TB Prevention
Richard Voigt Program Development and Support Branch
Division of STD Prevention
National Center for HIV, STD, and TB Prevention
Christopher Chinn Strategic Product Development
SmithKline Beecham Pharmaceuticals
Margaret Cobb, M.D., Ph.D. Director, Medical Affairs
Glaxo Wellcome Inc.
Sean Cunliffe Director, Gastrointestinal/Anti-Viral Marketing
Glaxo Wellcome Inc.
Gray Davis, Ph.D. President
Herpes Advice Center
R. Mark Evans, Ph.D. Division of Continuing Medical Education
American Medical Association
Dr. James C. Farr Manager, Professional Relations/Medical Education
3M Pharmaceuticals
Miles Jones Produce Manager, Valtrex
Glaxo Wellcome Inc.
Michael Spector Product manager for Famvir
SmithKline Beecham Pharmaceuticals
Appendix 3
Selected HSV Type-Specific Antibody Assays Based on Glycoprotein
G
Test
Type
Antigen Source
Sens./Speci. for HSV-2
Comment
Western blot 13, 15
Research/Reference
Infected cell lysates (HSV-1 and HSV-2)
>99 >99
Tested against culture
Available in Seattle & Australia
HSV-1 and HSV-2 IgG Differentiation
Immunoblot
Commercial (MRL)
Recombinant gG-1 and gG-2
95 95
Tested in-house; In trials for FDA
Includes type-common protein
Immunodot enzyme assay (IEA) 13, 17
Research
Monoclonal antibody-selected gG-1, gG-2
98 100
Tested against culture
HSV-1 and HSV-2 gG ELISA 21
Commercial (Gull)
Monoclonal antibody-selected
Native gG-1 and gG-2
98 97
In trials for FDA
gG-1 and gG-2 capture ELISA 6
Research
Infected cell lysates (HSV-1 and HSV-2)
89 100
Tested against culture and IEA
Indirect gG-2 ELISA 25
Research/Reference
Lectin-selected
Native gG-2
98 >99
Tested against culture
Available in Australia
POCkit™ -HSV-2 26
Commercial
(Diagnology)
Lectin-selected
Native gG-2
94 98
In trials for FDA
Detects light chain
Cobas® Core HSV-2 IgG EIA 27
Commercial (Roche)
Lectin-selected
Native gG-2
98 98
Macrobead format
Tested in-house
Baculovirus gG immunoblot 28
Research
Baculovirus-recombinant gG-1 and gG-2
92 100
Tested against IEA
Centacor Captia Select HSV-2 gG
EIA 22
Commercial
Baculovirus-recombinant gG-2
90 99
Tested against Gull and Chiron tests
Europe only
Monoclonal antibody blocking RIA 7
Research/reference
Infected cell lysates (HSV-1 and HSV-2)
91 ND
Tested against culture
Available in UK
Rhoda L. Ashley 3-30-98
References for table above:
6. Hashido M, Lee FK, Inouye S et al. Detection of herpes simplex
virus type-specific antibodies by an enzyme-linked immunosorbent assay
based on glycoprotein G. J Med Virol 1997;53:319-323.
7. Slomka MJ, Ashley RL, Cowan RM et al. Monoclonal antibody blocking
tests for the detection of HSV-1 and HSV-2 specific humoral responses:
comparison with Western blot asay. J Virol Medth 1995;55:27-35.
13. Ashley RL, Militoni J, Lee F et al. Comparison of Western blot
(immunoblot) and glycoprotein G-specific immunodot enzyme assay for
detecting antibodies to herpes simplex virus types 1 & 2 in human
sera. J Clin Microbiol 1988;26:662-667.
15. Ho DWT, Field PR, Irving WL et al. Detection of immunoglobulin
M antibodies to glycoprotein G-2 by Western blot (Immunoblot) for diagnosis
of initial herpes simplex virus type 2 genital infections. J Clin Microbiol
1993;31:3157-3164.
17. Lee FK, Coleman RM, Pereira L et al. Detection of herpes simplex
virus type 2-specific antibody with glycoprotein G. J Clin Microbiol
1985;22:641-644.
21. Ashley RL, Wu L, Pickering JW et al. Premarket evaluation of a
commercial glycoprotein-G based enzyme immunoassay for herpes simplex
virus type-specific antibodies. J Clin Microbiol 1998;36:294-295.
22. Groen J, Van Dijk G, Niesters HGM et al. Comparison of two enzyme-linked
immunosorbent assays and one rapid immunoblot assay for detection of
herpes simplex virus type 2-specific antibodies in serum. J Clin Microbiol
1998;36:845-847.
25. Ho DWT, Field PR, Sjogren-Jansson E et al. Indirect ELISA for
the detection of HSV-2 specific IgG and IgM antibodies with glycoprotein
G (gG-2). J Virol Meth 1992;36:249-264.
26. Ashley R, Eagleton M. Evaluation of a novel point-of-care test
for antibodies to herpes simplex virus type 2. Sex Trans Inf (in press).
27. Enders G, Bartlet U, Risse B et al. Evaluation of an enzyme immunoassay
on the immunoanalyzer CobasRCore for the specific detection of IgG
antibodies to herpes simplex virus type 2. Clin Lab 1997;43:753-756.
28. Sanchez-Martinez D, Schmid DS, Whittington W et al. Evaluation
of a test based on baculovirus-expressed glycoprotein G for detection
of herpes simplex virus type-specific antibodies. J Infect Dis 1991;164;1196-1199.
