Human Rickettsia felis Infection, Canary Islands, Appendix

Past Issue

Vol. 11, No. 12
December 2005

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Dispatch

Human Rickettsia felis Infection, Canary Islands, Spain

Jose-Luis Pérez-Arellano,*† Florence Fenollar,‡ Alfonso Angel-Moreno,*† Margarita Bolaños,*† Michele Hernández,*† Evora Santana,*† Marion Hemmersbach-Miller,*† Antonio-M Martín,*† and Didier Raoult‡
*Hospital Universitario Insular de Las Palmas, Canary Islands, Spain; †Universidad de Las Palmas de Gran Canaria, Canary Islands, Spain; and ‡Université de la Mediterranée, Marseille, France

Appendix. Serologic Data on 31 Patients with Rickettsiosis*

Patient no.	Immunofluorescence serologic testing IgG/IgM										Tools of specific diagnosis

	Rc	Rmas	Rmog	Rslo	Rhel	Raes	Raf	Rfel	Rt	Rpro

R. felis infection
1	0/1:128	0/1:128	0/1:128	0/1:128	0/1:128	0/1:64	0/1:64	1:64/1:256	1:64/1:256	1:32/1:256	Western blot using adsorbed sera
2	0/1:128	0/1:128	0/1:128	0/1:128	0/1:128	0/1:128	0/1:128	1:512/1:256	1:512/1:256	1:512/1:256	Western blot using adsorbed sera
3	1:16/1:256	1:16/1:256	1:16/1:256	1:16/1:256	1:16/1:256	1:16/1:16	1:16/1:16	1:32/1:64	1:32/1:64	1:32/1:64	Western blot using adsorbed sera
4	1:32/1:256	1:32/1:256	1:32/1:256	1:32/1:256	1:32/1:256	0/1:256	0/1:256	121:8/1:256	121:8/1:256	121:8/1:256	Western blot using adsorbed sera
5	1:256/1:128	1:256/1:128	1:256/1:128	1:256/1:128	1:256/1:128	1:64/1:64	121:8/1:64	1:256/1:64	1:256/1:64	1:256/1:64	Western blot using adsorbed sera
Murine typus
6	0/1:128	0/1:128	0/1:128	0/1:32	0/1:128	0/1:64	0/1:64	1:64/1:128	1:256/1:128	1:64/1:128	Dilution test
7	1:32/1:16	1:32/1:16	0/1:16	1:32/1:16	1:32/1:16	0/0	0/0	1:32/1:256	1:32/1:256	1:32/1:256	Western blot
8	0/1:8	0/1:8	0/1:8	0/1:8	0/1:8	0/0	0/0	1:32/0	121:8/0	1:32/0	Dilution test
9	0/1:8	0/1:8	0/1:8	0/1:8	0/1:8	0/0	0/0	1:32/0	1:256/0	1:32/0	Dilution test
10	0/1:64	0/1:64	0/1:64	0/1:64	0/1:64	0/1:8	0/1:8	1:64/1:512	1:64/1:512	0/1:512	Western blot using adsorbed sera
11	0/1:8	0/1:8	0/1:8	0/1:8	0/1:8	0/1:8	0/1:8	1:64/1:256	1:32/1:256	0/1:256	Western blot using adsorbed sera
12	0/1:64	0/1:64	0/1:64	0/1:64	0/1:64	0/0	0/0	1:256/1:256	121:8/1:256	121:8/1:256	Western blot
13	0/1:128	0/1:128	0/1:128	0/1:128	0/1:128	0/1:128	0/1:128	1:32/1:256	121:8/1:256	1:32/1:256	Dilution test
14	0/0	0/0	0/0	0/0	0/0	0/1:64	0/1:64	0/1:8	1:256/1:64	12/1:64	Dilution test
15	0/0	0/0	0/0	0/0	0/0	0/0	0/0	0/1:32	1:512/1:32	1:256/1:16	Dilution test
16	1:32/1:32	1:32/1:32	1:32/1:32	1:32/1:32	1:32/1:32	1:32/1:16	1:32/1:16	1:256/1:32	1:256/1:32	1:256/1:32	Western blot using adsorbed sera
17	0/1:128	0/1:128	0/1:128	0/1:128	0/1:128	0/1:32	0/1:64	1:16/1:128	1:32/1:128	1:32/1:128	Western blot using adsorbed sera
18	1:16/1:64	12/1:64	1:16/1:64	1:16/1:64	1:16/1:64	0/1:32	0/1:64	1:256/1:64	1:512/1:64	1:256/1:64	Western blot using adsorbed sera
Indeterminate rickettsiosis
19	0/0	0/0	0/0	0/0	0/0	0/0	0/0	0/1:16	0/1:64	0/1:64	None
20	0/1:64	0/1:64	0/1:64	0/1:64	0/1:64	0/1:8	0/1:8	0/1:64	0/1:256	0/1:256	None
21	1:64/0	1:64/0	1:64/0	1:64/0	1:64/0	0/0	0/0	1:32/0	*1:256/0*	121:8/0	None
22	1:16/0	1:16/0	1:16/0	1:16/0	1:16/0	1:8/0	1:8/0	1:64/0	1:64/0	0/0	None
23	0/0	0/0	0/0	0/0	0/0	0/0	0/0	0/1:64	0/1:256	0/1:256	None
24†	0/1:32	0/1:32	0/1:32	0/1:32	0/1:32	0/0	0/0	0/0	0/0	0/0	None
25†	0/1:64	0/1:64	0/1:64	0/1:64	0/1:64	0/0	0/0	0/0	0/0	0/0	None
26	0/1:16	0/1:16	0/1:16	0/1:16	0/1:16	0/1:16	0/1:16	121:8/0	*1:256/1:16*	*1:256/1:16*	None
27	0/1:32	0/1:32	0/1:32	0/1:32	0/1:32	0/1:16	0/1:16	0/1:16	0/1:64	0/1:64	None
28	0/0	0/0	0/0	0/0	0/0	0/0	0/0	0/1:16	1:64/1:16	1:64/1:16	None
29	1:32/1:256	1:32/1:256	1:32/1:256	1:32/1:256	1:32/1:256	1:32/1:256	1:32/1:256	1:32/1:256	1:256/1:256	1:256/1:256	None
30	0/1:16	0/1:16	0/1:16	0/1:16	0/1:16	0/1:16	0/1:8	1:16/1:16	1:32/1:32	1:32/1:32	None
31	0/1:128	0/1:128	0/1:128	0/1:128	0/1:128	0/1:16	0/1:128	1:32/1:128	1:256/1:256	1:256/1:256	None

IgG, immunoglobulin G; IgM, immunoglobulin M; Rc, Rickettsia conorii; Rmas, R. massilliae; Rmog, R. mongolotimonae; Rslo, R. slovaca; Rhel, R. helvetica; Raes, R. aeschlimannii; Raf, R. africae; Rfel, R. felis; Rt, R. typhi; Rpro, R. prowazekii*.
When the difference in titer between several antigens was <2 dilutions, the serologic data are in bold.
The serologic titers in italics indicate the antigens tested for the patients by using Western blot and cross-adsorptions.
†For these 2 patients, Western blot and cross-adsorptions were not performed.

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D. Raoult, Unité des Rickettsies, CNRS UMR 6020, Faculté de Médecine, Université de la Méditerranée, 27 Bd Jean Moulin, 13385 Marseille CEDEX 05, France; fax: 33-4-91-38-77-72; email: Didier.Raoult@medecine.univ-mrs.fr

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