CDC - Characterization of Flagella Produced by Clinical Strains of Stenotrophomonas maltophilia

Volume 8, Number 9, September 2002

Research

Characterization of Flagella Produced by Clinical Strains of Stenotrophomonas maltophilia

Doroti de Oliveira-Garcia,*† Monique Dall'Agnol,‡ Mónica Rosales,‡ Ana C.G.S. Azzuz,§ Marina B. Martinez,†§ and Jorge A. Girón‡
*Laboratório Clínico do Instituto Dante Pazzanese de Cardiologia, São Paulo, Brazil; †Departamento de Microbiologia do Instituto de Ciências Biomédicas da Universidade de São Paulo, São Paulo, Brazil; ‡Benemerita Universidad Autónoma de Puebla, Puebla, México; and §Departamento de Análises Clínicas da Faculdade de Ciências Farmacêuticas da Universidade de São Paulo, São Paulo, Brazil


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	Figure 2. Analysis of flagella purified from SMDP92. (A) sodium dodecyl-sulfate polyacrylamide gel electrophoresis of purified flagella, showing the 38-kDa flagellin subunit. Lane 1, molecular weight standards; lane 2, purified SM_FliC. (B) Immunoblotting and reactivity of purified flagella with anti-SM_FliCantibodies. The 38-kDa flagellin is indicated by an arrow. (C) Electron microscopy of purified flagella visualized by negative staining. Bar, 0.37 mm.

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