Volume 12, Number 3, March 2006

Identifying and Quantifying Genotypes in Polyclonal Infections due to Single Species

James M. Colborn,* Ousmane A. Koita,† Ousmane Cissé,† Mamadou W. Bagayoko,† Edward J. Guthrie,‡ and Donald J. Krogstad*
*Tulane University Health Sciences Center, New Orleans, Louisiana, USA; †University of Bamako, Bamako, Mali; and ‡Agilent Technologies, Wilmington, Delaware, USA

Figure 1. Use of capillary electrophoresis to identify multiple genotypes within single allotypes amplified by real-time polymerase chain reaction. Panel A shows the relative fluorescence values for 3 samples from infected patients by using primers specific for the K1 allotype of merozoite surface protein 1 (msp1). Panels B, C, and D show that those samples contained 3, 4, and 1 different K1 genotype parasites, respectively, identified by amplicons of 106, 124, and 142 bp (panel B), 105, 124, 142, and 160 bp (panel C), and 160 bp (panel D), respectively. The first and last peaks on each electropherogram are the 15- and 600-bp standard markers used to define the sizes of the unknown amplicons.

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This page last reviewed February 14, 2006

Emerging Infectious Diseases Journal
National Center for Infectious Diseases
Centers for Disease Control and Prevention