Volume 10, Number 7, July 2004

Detection of SARS-associated Coronavirus in Throat Wash and Saliva in Early Diagnosis

Wei-Kung Wang,*† Shey-Ying Chen,† I-Jung Liu,* Yee-Chun Chen,† Hui-Ling Chen,* Chao-Fu Yang,* Pei-Jer Chen,* Shiou-Hwei Yeh,‡ Chuan-Liang Kao,* Li-Min Huang,† Po-Ren Hsueh,† Jann-Tay Wang,† Wang-Hwei Sheng,† Chi-Tai Fang,† Chien-Ching Hung,† Szu-Min Hsieh,† Chan-Ping Su,† Wen-Chu Chiang,† Jyh-Yuan Yang,§ Jih-Hui Lin,§ Szu-Chia Hsieh,* Hsien-Ping Hu,* Yu-Ping Chiang,* Jin-Town Wang,* Pan-Chyr Yang,† Shan-Chwen Chang,† and members of the SARS Research Group of the National Taiwan University/National Taiwan University Hospital
*National Taiwan University, Taipei, Taiwan; †National Taiwan University Hospital, Taipei, Taiwan; ‡National Health Research Institute, Taipei, Taiwan; and §Center for Disease Control, Taipei, Taiwan

Figure 1. Quantification of the severe acute respiratory syndrome–associated coronavirus (SARS-CoV) RNA by real-time reverse transcription–polymerase chain reaction (RT-PCR) assay. (A) Location of the forward and reverse primers and probe in the genome of SARS-CoV, with the genome positions shown according to the Urbani strain (20). (B) A schematic diagram of the construct, ORF1b/pCRII-TOPO, and the protocol for generating the in vitro transcribed RNA as the standard for the real-time RT-PCR assay is shown. The relationship between known input RNA copies to the threshold cycle (CT) is shown at the bottom.

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This page last reviewed June 16, 2004

Emerging Infectious Diseases Journal
National Center for Infectious Diseases
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